Expression of Claudin-2 in intestinal metaplastic mucosa of Cdx2-transgenic mouse stomach
Expression of Claudin-2 in intestinal metaplastic mucosa of Cdx2-transgenic mouse stomach
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DOI：
10.3109/00365521.2010.501522
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发表时间：
2010-10
影响因子：
1.9
通讯作者：
H. Sakamoto;H. Mutoh;K. Sugano
中科院分区：
文献类型：
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作者：
H. Sakamoto;H. Mutoh;K. Sugano
抽象目标。CDX2在人的肠化生黏膜中表达，并在转基因小鼠胃中诱导肠化生黏膜。Claudin-2是肠道紧密连接的结构成分，在人类肠道上皮细胞系Caco-2中，Cdx2激活了Claudin-2启动子。本研究的目的是探讨Claudin-2基因在转基因小鼠胃肠化生黏膜中的表达。材料和方法。用免疫组织化学、Western blotting和实时定量聚合酶链式反应(qRT-PCR)分析Claudin-2在野生型小鼠正常胃粘膜、正常肠粘膜和转基因小鼠肠上皮化生组织中的表达。结果。Claudin-2在肠化生和肠化生中表达于腺体底部，在胃体中不表达。Claudin-2在胃窦部表达较弱，在肠化生和肠化生中表达较弱。肠上皮化生、结肠和回肠组织中均可检测到claudin-2，而胃体中未检测到claudin-2。结论。这些结果表明，CDX2在Claudin-2的体内表达中起着重要作用。
Abstract Objective. Cdx2 is expressed in human intestinal metaplastic mucosa and induces intestinal metaplastic mucosa in Cdx2-transgenic mouse stomach. Claudin-2 is a structural component of tight junctions in the intestine and Cdx2 activates the Claudin-2 promoter in the human intestinal epithelial cell line Caco-2. Our aim is to evaluate the expression of Claudin-2 in intestinal metaplastic mucosa of Cdx2-transgenic mouse stomach. Material and methods. The Claudin-2 expression in the normal gastric mucosa and normal intestinal mucosa of wild type mice and the intestinal metaplastic mucosa of Cdx2-transgenic mice was analyzed by immunohistochemistry, Western blotting and quantitative real-time PCR (qRT-PCR). Results. Claudin-2 was expressed in the base of the glands in intestine and intestinal metaplasia while it was not expressed in the body of stomach. Claudin-2 expression was found in the antrum of stomach, while it was weaker than that in the intestine and the intestinal metaplasia. Claudin-2 was also detected in intestinal metaplasia, colon and ileum by both Western blotting and qRT-PCR while it was not detected in gastric body. Conclusion. These results suggest that Cdx2 plays an important role in the expression of Claudin-2 in vivo.