Efficient transposition of the piggyBac resource (PB) transposon in mammalian cells and mice
Efficient transposition of the piggyBac resource (PB) transposon in mammalian cells and mice
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DOI：
10.1016/j.cell.2005.07.013
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发表时间：
2005-08-12
期刊：
影响因子：
64.5
通讯作者：
Xu, T
中科院分区：
文献类型：
--
作者：
Ding, S;Wu, XH;Xu, T
转座因子在低等生物的遗传操作中有着广泛的应用，包括转基因动物的产生和插入突变。相反，由于缺乏有效的转座系统，转座子在小鼠和其他脊椎动物系统中的使用仍然受到限制。我们已经测试了piggyBac（PB），一个来自甘蓝夜蛾Trichoplusia ni的DNA转座子，在哺乳动物系统中转座的能力。我们表明，携带多个基因的PB元件可以有效地转座在人类和小鼠细胞系，也在小鼠。PB允许其携带的标记基因的表达。在生殖系转座过程中，PB可以精确地从原始插入位点切除并转座到小鼠基因组的不同位置，优选转录单位。这些数据提供了第一个和关键的一步，对一个高效的转座子系统的各种遗传操作，包括转基因和插入突变的小鼠和其他脊椎动物。
Transposable elements have been routinely used for genetic manipulation in lower organisms, including generating transgenic animals and insertional mutagenesis. In contrast, the usage of transposons in mice and other vertebrate systems is still limited due to the lack of an efficient transposition system. We have tested the ability of piggyBac (PB), a DNA transposon from the cabbage looper moth Trichoplusia ni, to transpose in mammalian systems. We show that PB elements carrying multiple genes can efficiently transpose in human and mouse cell lines and also in mice. PB permits the expression of the marker genes it carried. During germline transposition, PB could excise precisely from original insertion sites and transpose into the mouse genome at diverse locations, preferably transcription units. These data provide a first and critical step toward a highly efficient transposon system for a variety of genetic manipulations including transgenesis and insertional mutagenesis in mice and other vertebrates.