Polymorphism in the neurofibromin gene, Nf1, is associated with antagonistic selection on wing size and development time in Drosophila melanogaster.
Polymorphism in the neurofibromin gene, Nf1, is associated with antagonistic selection on wing size and development time in Drosophila melanogaster.
复制标题
DOI：
10.1111/mec.12301
复制
发表时间：
2013-05
影响因子：
4.9
通讯作者：
Hoffmann AA
中科院分区：
文献类型：
--
作者：
Lee SF;Eyre-Walker YC;Rane RV;Reuter C;Vinti G;Rako L;Partridge L;Hoffmann AA
在许多无脊椎动物中，身体大小显示出基于遗传的倾向，在寒冷的气候中尺寸会增加。大的身体尺寸通常与延长的发育时间相关。我们认为，在中枢神经系统特异性基因神经纤维蛋白1（NF 1）的变化及其与身体大小和发育时间。我们在自然人群中发现了两种主要的Nf 1单倍型，Nf 1-插入-A和Nf 1-缺失-G。这些单倍型的特征在于Nf 1内含子2中的45个碱基插入/缺失（INDEL）和A/G同义取代（位点L17277）。INDEL和邻近位点之间的连锁不平衡（LD）是高的，但似乎是限制在Nf 1基因间隔。在澳大利亚，Nf 1插入-A单倍型的频率随着纬度的增加而增加，其中翅膀尺寸较大，独立于染色体倒位在（3R）佩恩。出乎意料的是，Nf 1-插入-A单倍型与翅膀大小呈负相关。我们发现Nf 1-插入-A单倍型在发育时间较短的女性中富集。这表明Nf 1单倍型渐变群可能是由发育时间而不是大小的选择驱动的;来自南方（高纬度）的雌性D。黑腹果蝇种群保持快速发展的时间，尽管是相对较大的，和较高的发病率Nf 1-插入-A在澳大利亚南部可能有助于这种模式，而影响的Nf 1单倍型的大小可能会抵消其他位点的拮抗作用的大小和发展时间。我们的研究结果指出，潜在的复杂性，参与识别选择的遗传变异表现出多效性的影响时，研究是基于空间格局或关联研究。
In many invertebrates, body size shows genetically based clines, with size increasing in colder climates. Large body size is typically associated with prolonged development times. We consider variation in the CNS-specific gene neurofibromin 1 (Nf1) and its association with body size and development time. We identified two major Nf1 haplotypes in natural populations, Nf1-insertion-A and Nf1-deletion-G. These haplotypes are characterized by a 45-base insertion/deletion (INDEL) in Nf1 intron 2 and an A/G synonymous substitution (locus L17277). Linkage disequilibrium (LD) between the INDEL and adjacent sites is high but appears to be restricted within the Nf1 gene interval. In Australia, the frequency of the Nf1-insertion-A haplotype increases with latitude where wing size is larger, independent of the chromosomal inversion In(3R)Payne. Unexpectedly, the Nf1-insertion-A haplotype is negatively associated with wing size. We found that the Nf1-insertion-A haplotype is enriched in females with shorter development time. This suggests that the Nf1 haplotype cline may be driven by selection for development time rather than size; females from southern (higher latitude) D. melanogaster populations maintain a rapid development time despite being relatively larger, and the higher incidence of Nf1-insertion-A in southern Australia may contribute to this pattern whereas the effects of the Nf1 haplotypes on size may be countered by other loci with antagonistic effects on size and development time. Our results point to the potential complexity involved in identifying selection on genetic variants exhibiting pleiotropic effects when studies are based on spatial patterns or association studies.