Reduced expression of major royal jelly protein 1 gene in the mushroom bodies of worker honeybees with reduced learning ability
Reduced expression of major royal jelly protein 1 gene in the mushroom bodies of worker honeybees with reduced learning ability
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DOI：
10.1051/apido/2009075
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发表时间：
2010-03-01
期刊：
影响因子：
2.4
通讯作者：
Sasaki, Masami
中科院分区：
文献类型：
--
作者：
Hojo, Masaru;Kagami, Takahiro;Sasaki, Masami
欧洲蜜蜂的学习能力随着年龄的增长而发展。然而，当工蜂与它们的群体隔离并且仅喂食蔗糖溶液时，它们的学习发育受到阻碍。这种饲养方法使我们能够比较年龄相同但学习能力不同的工蜂。在这项研究中，我们研究了这种饲养条件对蘑菇体基因表达的影响，蘑菇体是昆虫大脑中参与学习和记忆的中心。差异显示实验比较了保持在蜂箱中的工蜂和隔离饲养的工蜂，结果显示隔离工蜂中主要皇家浆蛋白（mrjp）1基因的表达降低。MRJP1在下咽腺中合成，并在幼虫和蜂王食物中发挥营养功能。我们的研究结果表明，mrjp1在大脑功能中也很重要，可能与学习能力的发展有关。
The learning ability of European honeybees, Apis mellifera, develops with age. However, when worker bees are isolated from their colony and are fed only sucrose solution, their learning development is hindered. This rearing method has allowed us to compare worker bees of the same age but with different learning abilities. In this study, we examined the influence of this rearing condition on gene expression in the mushroom body, which is the insect brain center involved in learning and memory. A differential display experiment comparing worker bees maintained in a hive with those reared in isolation showed that the expression of the major royal jelly protein (mrjp) 1 gene was reduced in the isolated worker bees. MRJP1 is synthesized in the hypopharyngeal gland and serves a nutritional function in larval and queen food. Our results suggest that mrjp1 is also important in brain function, possibly involved in the development of learning ability.