Hepatitis B Virus Replication Could Enhance Regulatory T Cell Activity by Producing Soluble Heat Shock Protein 60 From Hepatocytes
Hepatitis B Virus Replication Could Enhance Regulatory T Cell Activity by Producing Soluble Heat Shock Protein 60 From Hepatocytes
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DOI：
10.1086/653496
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发表时间：
2010-07-15
影响因子：
6.4
通讯作者：
Shimosegawa, Tooru
中科院分区：
文献类型：
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作者：
Kondo, Yasuteru;Ueno, Yoshiyuki;Shimosegawa, Tooru
背景HBcAg特异性调节性T（T（reg））细胞在慢性B型肝炎的发病机制中起重要作用。可溶性热休克蛋白，尤其是可溶性热休克蛋白60（sHSP 60），可通过Toll样受体影响Treg细胞的功能。我们分析了可溶性热休克蛋白的产生和B型肝炎病毒（HBV）复制与两个临床样本从HBeAg阳性的慢性肝炎B患者（n = 24）和HBeAg阳性的慢性肝炎B患者（n = 24）和体外HBV复制的肝细胞。随后，我们用分离的T（reg）细胞检测了sHSP 60的生物学效应。慢性B型肝炎患者血清sHSP 60水平显著高于慢性丙型肝炎患者（P
Background. HBcAg-specific regulatory T (T(reg)) cells play an important role in the pathogenesis of chronic hepatitis B. Soluble heat shock proteins, especially soluble heat shock protein 60 (sHSP60), could affect the function of Treg cells via Toll-like receptor.Methods. We analyzed the relationship between soluble heat shock protein production and hepatitis B virus (HBV) replication with both clinical samples from HBeAg-positive patients with chronic hepatitis B (n = 24) and HBeAb-positive patients with chronic hepatitis B (n = 24) and in vitro HBV-replicating hepatocytes. Thereafter, we examined the biological effects of sHSP60 with isolated T(reg) cells.Results. The serum levels of sHSP60 in patients with chronic hepatitis B were statistically significantly higher than those in patients with chronic hepatitis C (P