Characterization of commercial antibodies for use in high resolution apo(a) phenotyping by immunoblot analysis.
Characterization of commercial antibodies for use in high resolution apo(a) phenotyping by immunoblot analysis.
复制标题
通过免疫印迹分析对用于高分辨率 apo(a) 表型分析的商业抗体进行表征。
DOI：
10.1016/0009-8981(95)06132-8
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Chiesa,G
中科院分区：
文献类型：
--
作者：
Gaw,A;Chiesa,G
高分辨率载脂蛋白（a）表型分型与载脂蛋白（a）基因分型结合使用，以阐明血浆脂蛋白（a）（Lp（a））水平控制的潜在机制。随着人们对这一领域的兴趣日益浓厚，越来越多的实验室正在建立这种方法。此前曾报道过使用内部单克隆抗体进行高分辨率apo（a）表型分析。在此证明了市售单克隆抗体的有效使用。比较两种一抗，并使用免疫印迹法进行载脂蛋白（a）亚型分析。当加载等量的Lp（a）时，无论载脂蛋白（a）亚型大小如何，均获得大致相等的信号强度。使用具有已知Lp（a）水平和单一表达apo（a）同种型的样品的系列稀释液来确定系统的检测限。两种单克隆抗体均可检测到0.05 mg/dl Lp（a）。
High resolution apo(a) phenotyping has been used in conjunction with apo(a) genotyping to elucidate underlying mechanisms of plasma lipoprotein(a) (Lp(a)) level control. With increasing interest in this field more laboratories are now establishing such methods. High resolution apo(a) phenotyping has previously been reported with in-house monoclonal antibodies. Here the effective use of a commercially available monoclonal antibody is demonstrated. Two primary antibodies were compared and apo(a) isoform analysis was performed using an immunobloltting method. When equal quantities of Lp(a) were loaded approximately equal signal intensities were obtained, irrespective of apo(a) isoform size. Serial dilutions of samples with known Lp(a) level and a single expressing apo(a) isoform were used to determine the detection limit of the system. With both monoclonals it was possible to detect 0.05 mg/dl Lp(a).