In vitro osteogenesis assays: Influence of the primary cell source on alkaline phosphatase activity and mineralization
In vitro osteogenesis assays: Influence of the primary cell source on alkaline phosphatase activity and mineralization
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DOI：
10.1016/j.patbio.2008.06.004
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发表时间：
2009-06-01
影响因子：
--
通讯作者：
McKee, M. D.
中科院分区：
文献类型：
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作者：
Hoemann, C. D.;El-Gabalawy, H.;McKee, M. D.
在体内的骨小梁骨折修复中，成骨是通过低氧条件下的软骨内成骨或血管附近的编织骨沉积而发生的。体外成骨试验通常用于测试成骨细胞对药物、激素以及骨和软骨修复用生物材料的反应。这些细胞培养模型概括了编织骨合成中发生的事件，并使用原代成骨细胞、成骨前体细胞，如骨髓来源的间充质基质细胞(BMSCs)或各种成骨细胞系进行。随着培养时间的延长，通常通过检测碱性磷酸酶(早期成骨细胞标记物)的活性水平和评估矿化的含有I型胶原的纤维细胞外基质的组装来评估细胞分化。在这篇综述中，我们对已发表的使用头盖骨细胞、颅骨来源细胞系和骨髓基质细胞的成骨分析进行了比较分析。在所有这些类型的细胞中，在各种成骨和矿化介质条件下，碱性磷酸酶活性随时间的变化相似；然而，碱性磷酸酶活性的水平与观察到的矿化水平并不成正比。(C)2008年爱思唯尔·马森公司。版权所有。
In trabecular bone fracture repair in vivo, osteogenesis occurs through endochondral ossification under hypoxic conditions, or through woven bone deposition in the vicinity of blood vessels. In vitro osteogenesis assays are routinely used to test osteoblastic responses to drugs, hormones, and biomaterials for bone and cartilage repair applications. These cell culture models recapitulate events that occur in woven bone synthesis, and are carried out using primary osteoblasts, osteoblast precursors such as bone marrow-derived mesenchymal stromal cells (BMSCs), or various osteoblast cell lines. With time in culture, cell differentiation is typically assessed by examining levels of alkaline phosphatase activity (an early osteoblast marker) and by evaluating the assembly of a collagen (type I)-containing fibrillar extracellular matrix that mineralizes. In this review, we have made a comparative analysis of published osteogenic assays using calvarial cells, calvaria-derived cell lines, and bone marrow stromal cells. In all of these cell types, alkaline phosphatase activity shows similar progression over time using a variety of osteogenic and mineralizing media conditions; however, levels of alkaline phosphatase activity are not proportional to observed mineralization levels. (C) 2008 Elsevier Masson SAS. All rights reserved.