MECHANISTIC ASPECTS OF THE ELECTROCHEMICAL OXIDATION OF DIHYDRONICOTINAMIDE ADENINE-DINUCLEOTIDE (NADH)
MECHANISTIC ASPECTS OF THE ELECTROCHEMICAL OXIDATION OF DIHYDRONICOTINAMIDE ADENINE-DINUCLEOTIDE (NADH)
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DOI：
10.1021/ja00541a024
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发表时间：
1980-01-01
影响因子：
15
通讯作者：
ELVING, PJ
中科院分区：
文献类型：
--
作者：
MOIROUX, J;ELVING, PJ
本文研究了NADH的单阶段阳极氧化过程，包括去除2个电子和1个质子形成NAD+，特别注意去质子化步骤及其与初始电位决定性电子转移步骤的关系，主要是在水介质中的玻碳电极（GCE）上，补充研究是在水介质中的热解石墨和铂电极上以及在Me_2SO中的GCE上;碳电极通常首先用吸附的NAD+层覆盖，以消除吸附控制的法拉第过程。电流函数、粘度校正扩散系数D和β的近似恒等式。值，指向基本上相似的溶质种类和电荷转移路径涉及不同的介质和不同的电极。D是. apprx。2倍。10-6在水溶液中为cm 2 s-1; k为aptx。60 s ~（-1）。
The apparently single stage anodic oxidation of NADH involving removal of 2 electrons and a proton to form NAD+ was examined with particular attention to the deprotonation step and its relationship to the initial potential-determining electron-transfer step, primarily at glassy carbon electrodes (GCE) in aqueous media with supplementary studies at pyrolytic graphite and platinum electrodes in aqueous media and at GCE in Me2SO; the carbon electrodes were generally first covered with an adsorbed NAD+ layer in order to eliminate adsorption-controlled faradaic processes. The near identities of current functions, viscosity-corrected diffusion coefficients D and .beta. values, point to essentially similar solute species and charge-transfer paths being involved in different media and at different electrodes. D is .apprx. 2 .times. 10-6 cm2 s-1 in aqueous solution; k is .apprx. 60 s-1 at the GCE covered with adsorbed NAD+.