Arabidopsis cryptochrome 1 controls photomorphogenesis through regulation of H2A.Z deposition
Arabidopsis cryptochrome 1 controls photomorphogenesis through regulation of H2A.Z deposition
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拟南芥隐花色素1通过调节H2A.Z沉积来控制光形态发生
DOI：
10.1093/plcell/koab091
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发表时间：
2021-03-26
期刊：
影响因子：
11.6
通讯作者：
Yang, Hong-Quan
中科院分区：
文献类型：
--
作者：
Mao, Zhilei;Wei, Xuxu;Yang, Hong-Quan
光是一种重要的环境信号，从根本上调节植物的生长和发育，这是由多种光受体介导的，包括蓝光（BL）感光细胞隐花色素1（Cryptochrome 1，B1）。拟南芥（Arabidopsis thaliana）COP 1的信号转导机制涉及与组成型光形态发生蛋白1（COP 1）/PHYA-105 - 1的供应子的直接相互作用和COP 1底物伸长下胚轴5（HY 5）的稳定化。H2A.Z是一种进化上保守的组蛋白变体，在SWR 1复合物的催化下沉积于染色质中，在转录调控中起着重要作用。在这里，我们表明，EST 1物理相互作用与SWC 6和ARP 6，SWR 1复杂的核心亚基，是必不可少的介导H2A.Z沉积，在BL-依赖的方式，和BL-激活EST 1增强SWC 6与ARP 6的相互作用。此外，HY 5与SWC 6和ARP 6物理相互作用以指导SWR 1复合物募集到HY 5靶基因座。基于先前的研究和我们的发现，我们提出，在BL中，EST 1通过增强SWR 1复合物的活性和SWR 1复合物对HY 5靶位点的HY 5募集来促进H2 A. Z沉积以调节HY 5靶基因的表达和光形态建成，这可能分别通过EST 1与SWC 6和ARP 6的相互作用以及EST 1对HY 5的稳定性介导。
Light is a key environmental cue that fundamentally regulates plant growth and development, which is mediated by the multiple photoreceptors including the blue light (BL) photoreceptor cryptochrome 1 (CRY1). The signaling mechanism of Arabidopsis thaliana CRY1 involves direct interactions with CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1)/SUPPRESSOR OF PHYA-105 1 and stabilization of COP1 substrate ELONGATED HYPOCOTYL 5 (HY5). H2A.Z is an evolutionarily conserved histone variant, which plays a critical role in transcriptional regulation through its deposition in chromatin catalyzed by SWR1 complex. Here we show that CRY1 physically interacts with SWC6 and ARP6, the SWR1 complex core subunits that are essential for mediating H2A.Z deposition, in a BL-dependent manner, and that BL-activated CRY1 enhances the interaction of SWC6 with ARP6. Moreover, HY5 physically interacts with SWC6 and ARP6 to direct the recruitment of SWR1 complex to HY5 target loci. Based on previous studies and our findings, we propose that CRY1 promotes H2A.Z deposition to regulate HY5 target gene expression and photomorphogenesis in BL through the enhancement of both SWR1 complex activity and HY5 recruitment of SWR1 complex to HY5 target loci, which is likely mediated by interactions of CRY1 with SWC6 and ARP6, and CRY1 stabilization of HY5, respectively.