Loliolide, isolated from Sargassum horneri; abate LPS-induced inflammation via TLR mediated NF-KB, MAPK pathways in macrophages
Loliolide, isolated from Sargassum horneri; abate LPS-induced inflammation via TLR mediated NF-KB, MAPK pathways in macrophages
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DOI：
10.1016/j.algal.2021.102297
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发表时间：
2021-04-21
影响因子：
5.1
通讯作者：
Jeon, You-Jin
中科院分区：
文献类型：
--
作者：
Jayawardena, Thilina U.;Kim, Hyun-Soo;Jeon, You-Jin
在LPS刺激下，研究了来自韩国海岸丰富的霍氏马尾藻的次级代谢产物萜烯内酯; 6-羟基-4，4，7a-三甲基-5，6，7，7a-四氢苯并呋喃-2（4 H）酮（Lolipidine）在RAW 264.7巨噬细胞中的抗炎特性。通过HPCPC、HPLC-MS、GC-MS和NMR辅助分离和鉴定。NO产生下调的初步评估影响了炎症介质的进一步研究，例如：iNOS、考克斯-2和促炎细胞因子，包括PGE 2，其在loli治疗下显著下降。炎症是通过从细胞膜上识别外来物质而引发的;结合MAPK信号转导研究了toll样受体调节的NF-κ B。实验结果表明，loliprimidine的潜力，减轻炎症的巨噬细胞面临的LPS。因此，这为通过可持续利用丰富的天然来源海藻并利用其次级代谢产物来调解炎症性疾病提供了见解。
The secondary metabolite terpene lactone; 6-hydroxy-4,4,7a-trimethyl-5,6,7,7a-tetrahydrobenzofuran-2(4H)one; (Loliolide) from Sargassum horneri which is abundant on the shores of South Korea was investigated for its anti-inflammatory properties in RAW 264.7 macrophages under LPS stimulation. Isolation and identification were assisted by HPCPC, HPLC-MS, GC-MS, and NMR. Initial assessments of NO production downregulation influenced further studies of inflammatory mediators such that; iNOS, COX-2, and pro-inflammatory cytokines including PGE2 which were significantly declined with loliolide treatment. Inflammation is initiated via the identification of alien matter from the cell membrane; the toll-like receptor modulated NF-KB was investigated combined with MAPK signaling. Experimental outputs manifested the potential of loliolide to abate the inflammation in macrophages which were confronted by LPS. Hence, this provides insight to the mediation of inflammatory disorders through the sustainable use of abundant natural source marine algae and utilizing its secondary metabolites.