Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst’s frog (B. holsti).
Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst’s frog (B. holsti).
复制标题
奥顿蛙 (Babina subaspera) 中新微卫星位点的开发和表征以及同属物种霍尔斯特蛙 (B. holsti) 中的交叉扩增。
DOI：
10.1007/s12686-013-9981-x
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发表时间：
2013
影响因子：
1.1
通讯作者：
and Masayuki Sumida
中科院分区：
文献类型：
--
作者：
Ryosuke Kakehashi;Takeshi Igawa;Noriko Iwai;Etsuko Shoda-Kagaya;and Masayuki Sumida
奥顿蛙（Babina subaspera）和霍尔斯特蛙（Babina holsti）都是属于蛙科的濒危姐妹物种。为了对这些物种进行广泛的遗传和生态研究，我们分离并鉴定了8个新的Otton蛙微卫星位点，并验证了Holst蛙沿着8个先前报道的位点的交叉扩增。新分离位点的等位基因总数和期望杂合度分别为5 ~ 12和0.620 ~ 0.905。我们还证实了交叉扩增在4个新的位点和所有以前报道的位点在霍尔斯特蛙与相同水平的多态性的奥顿蛙。我们的研究结果表明，这些新的基因座将适用于不同规模的保护遗传学研究。
The Otton frog (Babina subaspera) and Holst’s frog (Babina holsti) are both endangered sister species belonging to family Ranidae. For extensive genetic and ecological research of these species, we isolated and characterized 8 new microsatellite loci of the Otton frog and validated cross-amplification in Holst’s frog along with 8 previously reported loci. The total number of alleles and the expected heterozygosity of newly isolated loci in the Otton frog population ranged from 5 to 12 and from 0.620 to 0.905, respectively. We also confirmed cross-amplification in 4 of the new loci and in all previously reported loci in Holst’s frog with the same level of polymorphism as the Otton frog. Our findings suggest that these novel loci will be applicable for conservation genetic studies across varying scales.