Integrated control-path design and error recovery in the synthesis of digital microfluidic lab-on-chip
Integrated control-path design and error recovery in the synthesis of digital microfluidic lab-on-chip
复制标题
数字微流控片上实验室合成中的集成控制路径设计和错误恢复
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
K. Chakrabarty
中科院分区：
文献类型：
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作者：
Yang Zhao;Tao Xu;K. Chakrabarty
数字微流体技术的最新进展已经引起了人们对用于生化分析的小型化芯片实验室设备的极大兴趣。从实验室协议的规范中，也出现了用于芯片实验室自动设计的合成工具。然而，这些工具都没有考虑控制流或解决从芯片上生物测定执行期间可能发生的流体错误中恢复的问题。我们提出了一种综合方法，将控制路径和错误恢复机制的设计中的数字微流控芯片实验室。基于误差传播估计，我们确定生物芯片合成过程中流体检查点的最佳位置。微控制器通过截取映射到软件程序的合成结果来协调基于控制流的生物测定的实施。现实生活中的生物测定应用程序作为案例研究，以评估所提出的设计方法。对于代表性的蛋白质测定，与基线芯片设计相比，具有控制路径的生物芯片可以在生物测定的实施期间发生错误时将完成时间减少30%。
Recent advances in digital microfluidics have led to tremendous interest in miniaturized lab-on-chip devices for biochemical analysis. Synthesis tools have also emerged for the automated design of lab-on-chip from the specifications of laboratory protocols. However, none of these tools consider control flow or address the problem of recovering from fluidic errors that can occur during on-chip bioassay execution. We present a synthesis method that incorporates control paths and an error-recovery mechanism in the design of a digital microfluidic lab-on-chip. Based on error-propagation estimates, we determine the best locations for fluidic checkpoints during biochip synthesis. A microcontroller coordinates the implementation of the control-flow-based bioassay by intercepting the synthesis results that are mapped to the software programs. Real-life bioassay applications are used as case studies to evaluate the proposed design method. For a representative protein assay, compared to a baseline chip design, the biochip with a control path can reduce the completion time by 30% when errors occur during the implementation of the bioassay.