A Quantitative Analysis of Mineral Loss and Shrinkage of in vitro Demineralized Human Root Surfaces
A Quantitative Analysis of Mineral Loss and Shrinkage of in vitro Demineralized Human Root Surfaces
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体外脱矿人根表面矿物质损失和收缩的定量分析
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
O. Fejerskov
中科院分区：
文献类型：
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作者：
J. M. T. Cate;B. Nyvad;Y. M. Plassche;O. Fejerskov
牙本质标本的脱矿速度比牙釉质快。虽然这一点被普遍接受，但在进行显微放射分析之前，根部病变的收缩会阻碍对根部病变形成速度的量化。这导致了对病变深度和总矿物质损失的严重低估。本文的目的是定量测定根损伤形成过程中的矿物质损失率，并确定干燥引起的根标本的收缩。将未萌出的牙根分别置于0.1mol/L乳酸盐缓冲液(pH=4.8)和0.2 mmol/L甲氧基二磷酸盐的脱矿系统中，分别处理4、11、22和44天。用定量放射学方法对牙根病变进行评价。对脱矿液进行分析，以确定根组织的溶出量。对这两组数据的比较表明，使用脱矿系统后，根部损伤可能缩小高达62%。将标本固定在固定剂中并不影响收缩。化学分析表明，矿物质流失与时间呈线性关系。从这项研究的数据集，开发了一个模型来补偿牙本质标本的收缩。这样，可以计算出四个脱钙时间的病变深度分别为130220320和530μm。这些数值与镜检确定的病变深度是一致的。
Demineralization of dentin specimens proceeds at a faster rate than that of enamel. Although this is generally accepted, a quantification of the rate of formation of root lesions is hampered by the shrinkage of the lesions when these are dried prior to microradiographic analysis.' This leads to a significant underestimation of the lesion depth and total mineral loss. The aim of this paper was to quantitate the rate of mineral loss during root lesion formation in vitro and to determine the shrinkage of root specimens as a result of drying. Unerupted roots of human teeth were subjected to a demineralizing system of 0.1 mol/L lactate buffer (pH = 4.8) with 0.2 mmol/L methanehydroxydiphosphonate during four, 11, 22, and 44 days. The root lesions were assessed by quantitative microradiography. The demineralizing solutions were analyzed to determine the amounts of root tissue dissolved. A comparison of these two sets of data showed that, with the demineralizing system used, root lesions may shrink up to 62%. Fixation of the specimens in fixative did not affect this shrinkage. Chemical analysis showed that mineral loss proceeded linearly with time. From the data-sets of this study, a model was developed to compensate for the shrinkage in the dentin specimens. In this way, it was possible to calculate the lesion depth at four demineralization times as being 130, 220, 320, and 530 μm, respectively. These values were in agreement with a microscopic determination of the lesion depth.