DIFFERENT RHODOPSIN MONOCLONAL-ANTIBODIES REVEAL DIFFERENT BINDING PATTERNS ON DEVELOPING AND ADULT-RAT RETINA
DIFFERENT RHODOPSIN MONOCLONAL-ANTIBODIES REVEAL DIFFERENT BINDING PATTERNS ON DEVELOPING AND ADULT-RAT RETINA
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DOI：
10.1177/35.11.3655327
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发表时间：
1987-11-01
影响因子：
3.2
通讯作者：
BARNSTABLE, CJ
中科院分区：
文献类型：
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作者：
HICKS, D;BARNSTABLE, CJ
我们使用了一组10个单克隆抗体，针对紫红质羧基、跨膜环和氨基端区域内不同的已鉴定肽序列，来标记早期产后和成年大鼠的视网膜。标记的强度，染色最初出现的年龄和标记的分布取决于抗体。大多数抗体在出生后第1天显示出可检测的标记，并最终观察到与细胞体以及光感受器的内外段结合。然而，一个氨基端和两个羧基端抗体直到出生后第5天才显示出可检测的标记，并且只能在细胞体区域短暂检测到。这些模式不能用结合位点的可及性、结合亲和力、固定伪影或交叉反应性来解释。结果表明，生理和实验参数可以改变构象活性分子（如视紫红质）的表观免疫细胞化学定位。结果还表明，在大鼠睁眼之前，视紫红质可以在视网膜感光器的几个不同区室中发生光依赖性构象变化。
We used a battery of 10 monoclonal antibodies directed against different identified peptide sequences within the carboxyl, transmembrane loop, and amino terminal regions of rhodopsin to label retinas from early postnatal and adult rats. Intensity of label, age of initial appearance of staining, and distribution of label varied depending on the antibody. Most antibodies showed detectable labeling at postnatal day 1, and were eventually observed binding to the cell bodies and the inner and outer segments of the photoreceptors. One amino terminal and two carboxyl terminal antibodies, however, showed no detectable labeling until postnatal day 5 and were only transiently detectable in the cell body region. These patterns cannot be explained by accessibility of binding site, binding affinity, fixation artifact, or crossreactivity. The results indicate that physiological and experimental parameters can alter the apparent immunocytochemical localization of conformationally active molecules such as rhodopsin. The results also suggest that rhodopsin can undergo light-dependent conformational changes in several different compartments within rat retinal photoreceptors before the time of eye opening.