Forward and Reverse Genetic Approaches for the Analysis of Vertebrate Development in the Zebrafish
Forward and Reverse Genetic Approaches for the Analysis of Vertebrate Development in the Zebrafish
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DOI：
10.1016/j.devcel.2011.06.007
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发表时间：
2011-07-19
影响因子：
11.8
通讯作者：
Wolfe, Scot A.
中科院分区：
文献类型：
--
作者：
Lawson, Nathan D.;Wolfe, Scot A.
简单的正向和反向遗传方法的发展推动了生物学中基因功能的反卷积。虽然这些技术的起源存在于单细胞或无脊椎生物的研究中，但在许多情况下，这些方法已应用于脊椎动物模型系统，以获得对胚胎发育过程中基因功能的有力见解。这一观点提供了对斑马鱼脊椎动物基因功能研究的主要正向和反向遗传方法的总结，斑马鱼已成为动物发育研究的既定模型。
The development of facile forward and reverse genetic approaches has propelled the deconvolution of gene function in biology. While the origins of these techniques reside in the study of single-cell or invertebrate organisms, in many cases these approaches have been applied to vertebrate model systems to gain powerful insights into gene function during embryonic development. This perspective provides a summary of the major forward and reverse genetic approaches that have contributed to the study of vertebrate gene function in zebrafish, which has become an established model for the study of animal development.