Effects of repetitive transcranial magnetic stimulation on adenosine triphosphate content and microtubule associated protein-2 expression after cerebral ischemia-reperfusion injury in rat brain
Effects of repetitive transcranial magnetic stimulation on adenosine triphosphate content and microtubule associated protein-2 expression after cerebral ischemia-reperfusion injury in rat brain
复制标题
重复经颅磁刺激对大鼠脑缺血再灌注损伤后三磷酸腺苷含量及微管相关蛋白2表达的影响
DOI：
10.1097/00029330-200807020-00012
复制
发表时间：
2008-07-20
影响因子：
6.1
通讯作者：
Cui Li-Ying
中科院分区：
文献类型：
--
作者：
Feng Hong-Lin;Yan Li;Cui Li-Ying
背景重复经颅磁刺激（rTMS）研究主要集中在精神疾病和帕金森病的治疗效果上。一些研究表明，rTMS可以保护短暂缺血诱导的迟发性神经元死亡，增强缺血条件下的长时程增强，并影响局部脑血流和代谢。本研究旨在观察重复经颅磁刺激（rTMS）对大鼠大脑中动脉闭塞（MCAO）再灌注后脑组织三磷酸腺苷（ATP）含量和微管相关蛋白2（MAP-2）表达的影响。将90只大鼠随机分为3组，每组30只。为了研究多个rTMS参数对ATP含量和MAP-2表达的影响，每组大鼠进一步分为6个亚组（每组5只）。分别于再灌注后1h、24 h和48 h处死大鼠，取脑组织检测ATP和MAP-2的含量。结果rTMS可显著增加缺血后脑组织ATP含量和MAP-2的表达（P < 0.01），不同rTMS参数对左侧纹状体ATP含量和MAP-2表达的影响不同。结论rTMS可引起脑损伤区ATP含量和MAP-2表达的显著增加，提示rTMS促进神经修复的生物学机制可能与ATP和MAP-2的共同调节有关。因此，rTMS可能成为缺血性脑血管病的潜在辅助治疗方法。
Background Repetitive transcranial magnetic stimulation (rTMS) research has mainly been focused on the therapeutic effect of psychiatric disorders and Parkinson's disease. A few studies have shown that rTMS might protect against delayed neuronal death induced by transient ischemia, enhance long-term potentiation in ischemic conditions and affect regional brain blood flow and metabolism. The aim of this study was to determine the effects of repetitive transcranial magnetic stimulation (rTMS) on adenosine triphosphate (ATP) content and microtubule associated protein-2 (MAP-2) expression in rat brain after middle cerebral artery occlusion (MCAO)/reperfusion.Methods To study the effects of different timecourses of rTMS on ATP content and MAP-2 expression, 90 rats were randomly divided into three groups (30 rats in each group). To study the effects of multiple rTMS parameters on ATP content and MAP-2 expression, the rats in each group were further divided into six subgroups (five rats each). The rats were sacrificed at 1-hour, 24-hour and 48-hour intervals after reperfusion, and the brain tissues were collected for the detection of ATP and MAP-2.Results rTMS could significantly increase ATP content and MAP-2 expression in the left brain following ischemic insult (P < 0.01) and different rTMS parameters had different effects on the ATP level and the MAP-2 expression in the left striatum. A high-frequency rTMS played an important role in MAP-2 expression and ATP preservation.Conclusions This study revealed that rTMS induced significant increase of ATP content and MAP-2 expression in the injured area of the brain, suggesting that the regulation of both ATP and MAP-2 may be involved in the biological mechanism of the effect of rTMS on neural recovery. Therefore, rTMS may become a potential adjunctive therapy for ischemic cerebrovascular disease.