Ultrastructural observation of electron irradiation damage of lamellar bone
Ultrastructural observation of electron irradiation damage of lamellar bone
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DOI：
10.1007/s10856-008-3651-7
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发表时间：
2009-04-01
影响因子：
3.7
通讯作者：
Kohn, D. H.
中科院分区：
文献类型：
--
作者：
Hong, S. I.;Hong, S. K.;Kohn, D. H.
用高分辨电子显微镜(HRTEM)原位观察了小鼠股骨板层骨的超微结构及电子辐照(200kV)对骨胶原和矿物质的影响。胶原原纤维的条带大多平行于骨骼的长轴排列，有些纤维条带在纵切面上倾斜。纵横切面的超微结构相似，支持板层骨的旋转胶合板结构。电子线照射后胶原纤维受损，矿物晶体粗化。连续的衍射环变得斑点状，环与背景之间的对比变得更加尖锐，进一步表明辐照后磷灰石晶体变粗，结晶度增加。辐照后未观察到新的晶相。对胶原的破坏和磷灰石晶体的粗化都会恶化骨骼的强度和完整性，并可能为接受放射治疗的患者提供骨折情况的洞察。
The ultrastructure of murine femoral lamellar bone and the effect of electron irradiation (200 kV) on collagen and mineral features were investigated using in situ high resolution transmission electron microscopy (HRTEM). Bands of collagen fibrils were mostly aligned parallel to the long axis of the bones, with some bands of fibrils inclined in longitudinal sections. The similarity of the ultrastructure between the longitudinal and transverse sections supports the rotated plywood structure of the lamellar bone. The collagen fibrils appeared damaged and the mineral crystals were coarsened after electron irradiation. Continuous diffraction rings became spotty and the contrast between rings and the background became sharper, further suggesting coarsening of apatite crystals and increased crystallinity after irradiation. No new phases were observed after irradiation. Both the damage to collagen and coarsening of apatite crystals can deteriorate the strength and integrity of bone, and may provide insight into fracture in patients who have undergone radiation therapy.