Crosslinked basement membrane-based coatings enhance glucose sensor function and continuous glucose monitoring in vivo.
Crosslinked basement membrane-based coatings enhance glucose sensor function and continuous glucose monitoring in vivo.
复制标题
基于交联基底膜的涂层增强了葡萄糖传感器功能和体内连续葡萄糖监测。
DOI：
10.1002/jbm.a.36206
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Kreutzer,DonaldL
中科院分区：
文献类型：
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作者：
Klueh,Ulrike;Ludzinska,Izabela;Czajkowski,Caroline;Qiao,Yi;Kreutzer,DonaldL
克服传感器诱导的组织反应是实现糖尿病管理中成功的连续血糖监测（CGM）的基本要素，特别是在闭环技术中使用时。最近，我们证明了基底膜（BM）为基础的葡萄糖传感器涂层可以显著减少设备植入部位的组织反应。然而，生物相容性BM基生物水凝胶传感器涂层在不到3周的时间内迅速降解，这有效地消除了保护性传感器涂层。为了提高BM涂层的稳定性和有效性，我们评估了使用戊二醛作为交联剂（指定为X‐Cultrex）交联BM的影响。对这些X‐Cultrex涂层在体外和体内的影响进行了传感器性能（未重新校准）评估。在小鼠CGM模型中对传感器性能进行了28天的评估，并以平均绝对相对差（MARD）值表示。采用标准组织学技术，在28天的时间周期内评估Cultrex包被、X - Cultrex包被和未包被葡萄糖传感器的组织反应性。这些研究表明，基于X - Cultrex的传感器涂层对体外葡萄糖传感器功能没有影响。在体内，在整个28天的研究中，X - Cultrex涂层显著增强了葡萄糖传感器的性能。与未涂覆的传感器相比，经X - Cultrex处理的传感器的组织学评估显示，其组织反应性明显降低。©2017 Wiley期刊公司中国生物医学工程学报，2016,31(1):444 - 444。
Overcoming sensor‐induced tissue reactions is an essential element of achieving successful continuous glucose monitoring (CGM) in the management of diabetes, particularly when used in closed loop technology. Recently, we demonstrated that basement membrane (BM)‐based glucose sensor coatings significantly reduced tissue reactions at sites of device implantation. However, the biocompatible BM‐based biohydrogel sensor coating rapidly degraded over a less than a 3‐week period, which effectively eliminated the protective sensor coating. In an effort to increase the stability and effectiveness of the BM coating, we evaluated the impact of crosslinking BM utilizing glutaraldehyde as a crosslinking agent, designated as X‐Cultrex. Sensor performance (nonrecalibrated) was evaluated for the impact of these X‐Cultrex coatingsin vitroandin vivo. Sensor performance was assessed over a 28‐day time period in a murine CGM model and expressed as mean absolute relative difference (MARD) values. Tissue reactivity of Cultrex‐coated, X‐Cultrex‐coated, and uncoated glucose sensors was evaluated over a 28‐day time periodin vivousing standard histological techniques. These studies demonstrated that X‐Cultrex‐based sensor coatings had no effect on glucose sensor functionin vitro. In vivo, glucose sensor performance was significantly enhanced following X‐Cultrex coating throughout the 28‐day study. Histological evaluations of X‐Cultrex‐treated sensors demonstrated significantly less tissue reactivity when compared to uncoated sensors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 7–16, 2018.