3-Tesla magnetic resonance angiographic assessment of a tissue-engineered small-caliber vascular graft implanted in a rat
3-Tesla magnetic resonance angiographic assessment of a tissue-engineered small-caliber vascular graft implanted in a rat
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对植入大鼠体内的组织工程小口径血管移植物进行 3-Tesla 磁共振血管造影评估
DOI：
10.1002/jbm.b.31501
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Nakayama Y
中科院分区：
文献类型：
--
作者：
Yamanami M;Yamamoto A;Iida H;Watanabe T;Kanda K;Yaku H;Nakayama Y
在小口径血管移植物（直径小于3 mm）的开发中，动物植入研究大多使用大鼠腹主动脉进行，其一定的通畅性必须通过牺牲每个观察期来评估，在进行大量实验时既费力又耗时。本研究首次证明了3 T无造影剂飞行时间磁共振血管造影（TOF-MRA）在大鼠组织工程血管移植物状态连续评估中的应用。作为模型移植物，使用通过将硅胶棒（直径; 1.5 mm）嵌入大鼠皮下袋中2周进行体内组织工程而制备的单个结缔管状组织（直径; 1.5 mm）。通过端对端吻合将移植物植入大鼠腹主动脉（直径：1.3 mm）。在植入后3个月随访期内获得的移植物的重复TOF-MRA成像使得可以简单且无创地评价移植物的通畅性。它还允许可视化连接的腹主动脉和肾动脉以及具有较小口径（直径;小于1 mm）的髂总动脉。此外，还可以检测狭窄或动脉瘤的程度。3 T MRA允许在同一只大鼠的不同时间对血管移植物的状态进行简化和非侵入性评估，包括狭窄或动脉瘤的形成，这将有助于促进组织工程血管移植物的发展，即使是小口径。© 2009 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 2010
In the development of small‐caliber vascular grafts (diameter; less than 3 mm), animal implantation studies have been mostly performed by using rat abdominal aortas, and their certain patency must evaluate with sacrificing every observation periods, which is both labor‐intensive and time‐consuming when performing a large number of experiments. This study is the first to demonstrate the application of 3‐Tesla contrast‐free time‐of‐flight magnetic resonance angiography (TOF‐MRA) in the continuous assessment of the status of a tissue‐engineered vascular graft in rat. As a model graft, a single connective tubular tissue (diameter; 1.5 mm), prepared by embedding the silicone rod (diameter; 1.5 mm) into a subcutaneous pouch of a rat for 2 weeks anin vivotissue‐engineering, was used. The graft was implanted in the abdominal aorta (diameter; 1.3 mm) of the rat by end‐to‐end anastomosis. Repeated TOF‐MRA imaging of the graft obtained over a 3‐month follow‐up period after implantation made it possible to evaluate the patency of the graft, both simply and noninvasively. It also permitted visualization of the connected abdominal aorta and renal and common iliac arteries having smaller caliber (diameter; less than 1 mm). In addition, the degree of the stenosis or aneurysm could also be detected. 3‐Tesla MRA allowed the simplified and noninvasive assessment of the status on the vascular graft, including the formation of a stenosis or aneurysm, in the same rat at different times, which will be contributing to enhance the development of tissue‐engineered vascular grafts even with small caliber. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2010