RIP: An mRNA Localization Technique
RIP: An mRNA Localization Technique
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DOI：
10.1007/978-1-61779-005-8_25
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发表时间：
2011-01-01
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影响因子：
--
通讯作者：
Tenenbaum, Scott A.
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文献类型：
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作者：
Jayaseelan, Sabarinath;Doyle, Francis;Tenenbaum, Scott A.
对转录后基因表达的详细理解对于关联参与协调细胞生物分子机制所需的许多水平的RNA-蛋白质相互作用的不同元件是必要的。mRNA的概况，这一机制的主要组成部分，可以从特定的RNA结合蛋白（RBP）分离后进行检查。RIP-Chip或ribonomic profiling是一种通用的体内技术，已被广泛用于研究转录后基因调控和mRNA定位。在这里，我们精心详细的mRNA分离的方法，使用RBP免疫沉淀（RIP）作为一种主要的方法。特异性抗体用于靶向RBP，然后用于捕获相关的mRNA。
A detailed understanding of post-transcriptional gene expression is necessary to correlate the different elements involved in the many levels of RNA-protein interactions that are needed to coordinate the cellular biomolecular machinery. The profile of mRNA, a major component of this machinery, can be examined after isolation from specific RNA-binding proteins (RBPs). RIP-Chip or ribonomic profiling is a versatile in vivo technique that has been widely used to study post-transcriptional gene regulation and the localization of mRNA. Here we elaborately detail the methodology for mRNA isolation using RBP immunoprecipitation (RIP) as a primary approach. Specific antibodies are used to target RBPs, which are then used to capture the associated mRNA.