Over-expression of human carcinoma-associated antigen in intrahepatic cholangiocellular carcinoma.
Over-expression of human carcinoma-associated antigen in intrahepatic cholangiocellular carcinoma.
复制标题
肝内胆管细胞癌中人癌相关抗原的过度表达。
DOI：
10.1016/j.bbrc.2011.01.040
复制
发表时间：
2011
影响因子：
3.1
通讯作者：
He
中科院分区：
文献类型：
--
作者：
Dong;Hao Wang;Hua;Shuang Li;H. Qu;X. Kou;Jing;Hui Wang;He
目的探讨人癌抗原（human carcinoma antigen，HCA）在肝内胆管细胞癌（intrahepatic cholangiocellular carcinoma，ICC）中的表达及其与临床特征的关系。对不同病例的低分化（n=20）、中-高分化（n=30）和高分化（n=10）肿瘤的区域进行了评估。结果BCE 075抗体和BDD 048抗体的染色模式相似。BCE 075的免疫阳性染色主要定位于细胞浆和细胞分泌物中。低分化ICC阳性率为15%，中高分化ICC阳性率为72%，高分化ICC阳性率为100%。但在邻近的正常组织中未检测到染色。这些组织中HCA表达的差异有统计学意义。此外，我们发现HCA的表达与ICC的分化程度和肿瘤细胞形态密切相关。HCA的表达与血清CA 19 - 9水平相关。结论HCA可作为胆管细胞癌诊断的潜在指标。
ObjectiveTo investigate the expression status of human carcinoma antigen (HCA) in human cholangiocellular carcinomas, and to determine the relationship between HCA and clinical features.MethodsTissues from 60 intrahepatic cholangiocellular carcinoma (ICC) patients, and normal liver tissues from 20 hepatic hemangioma patients selected randomly were assayed for the expression of HCA by immunohistochemistry, and Western blots. Areas of poorly differentiated (n=20), moderately-well differentiated (n=30), highly differentiated tumors (n=10) from different cases were evaluated. Results were recorded as positive (⩾5% of cells staining and staining intensity 2+ or 3+) or negative (<5% of cells staining and staining intensity <2+) and analyzed using the χ2test.ResultsBCE075 and BDD048 antibodies showed similar staining patterns. The positive immunostaining of BCE075 was mainly localized in the cytoplasm and cell secretions. The staining was positive in 15% of poorly differentiated ICC, 72% of moderately-well differentiated, 100% of highly differentiated tumors. But, staining was not detected in adjacent normal tissue. The differences in HCA expression among these tissues were statistically significant. Also, we found expression of HCA to be closely associated with the degree of differentiation of ICC and tumor cell morphology. There was a correlation between expression of HCA and serum CA19-9.ConclusionThe data suggest that HCA is a potential marker for the diagnosis of cholangiocellular carcinoma.