YEAST ORIGIN RECOGNITION COMPLEX FUNCTIONS IN TRANSCRIPTION SILENCING AND DNA-REPLICATION
YEAST ORIGIN RECOGNITION COMPLEX FUNCTIONS IN TRANSCRIPTION SILENCING AND DNA-REPLICATION
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DOI：
10.1126/science.8266072
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发表时间：
1993-12-17
期刊：
影响因子：
56.9
通讯作者：
STILLMAN, B
中科院分区：
文献类型：
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作者：
BELL, SP;KOBAYASHI, R;STILLMAN, B
已分离出编码酿酒酵母起源识别复合物（ORC）的两个亚基的基因。在编码ORC的72 kD亚基（ORC2）的基因中的温度敏感性突变的表征表明，这种蛋白质复合物在DNA复制过程的早期起作用。此外，来源于orc2ts细胞的ORC对于DNA结合是有缺陷的。其他研究显示orc2ts细胞在沉默交配型基因座的转录沉默中存在缺陷。与这一发现一致，ORC特异性结合酵母中鉴定的四种交配型沉默剂中的每一种。这些研究结果支持的假设，ORC作为一个启动蛋白在酵母DNA复制的起点，并建议ORC也在确定转录结构域的功能。
The genes encoding two of the subunits of the Saccharomyces cerevisiae origin recognition complex (ORC) have been isolated. Characterization of a temperature-sensitive mutation in the gene encoding the 72-kD subunit of ORC (ORC2) indicates that this protein complex functions early in the DNA replication process. Moreover, ORC derived from orc2ts cells is defective for DNA binding. Others have shown a defect in orc2ts cells in transcriptional silencing at the silent mating-type loci. Consistent with this finding, ORC specifically binds to each of the four mating-type silencers identified in yeast. These findings support the hypothesis that ORC acts as an initiator protein at yeast origins of DNA replication and suggest that ORC also functions in the determination of transcriptional domains.