Genomic basis for coral resilience to climate change
Genomic basis for coral resilience to climate change
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DOI：
10.1073/pnas.1210224110
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发表时间：
2013-01-22
影响因子：
11.1
通讯作者：
Palumbi, Stephen R.
中科院分区：
文献类型：
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作者：
Barshis, Daniel J.;Ladner, Jason T.;Palumbi, Stephen R.
DNA测序技术的最新进展现在允许深入表征模式分类群之外的许多生物体的基因组应激反应。它们特别适合造礁珊瑚等生物，随着人为气候变化的加剧，这些生物的丰度预计将急剧下降。不同的珊瑚在对环境压力的生理恢复力方面存在很大差异，但珊瑚恢复力增强背后的分子机制仍不清楚。在这里，我们比较了同种热敏感和热弹性珊瑚之间的转录组范围内的基因表达（通过使用Illumina测序的RNA-Seq），以确定有助于珊瑚弹性的分子途径。在模拟漂白胁迫下，敏感和弹性珊瑚改变了数百个基因的表达，但弹性珊瑚在控制条件下的60个基因表达更高。这些“前载”转录在热应激期间在弹性珊瑚中上调较少，包括热耐受性基因，如热休克蛋白和抗氧化酶，以及参与细胞凋亡调节、肿瘤抑制、先天免疫反应和细胞粘附的广泛基因。我们提出，组成前负荷使个人在经常遇到的环境压力，一个想法，有很强的相似之处，如酵母模型系统，以保持生理弹性。我们的研究为负责增强应激耐受性的基本细胞过程提供了广泛的见解，这可能使一些生物体能够在全球气候变化的时代更好地坚持到未来。
Recent advances in DNA-sequencing technologies now allow for in-depth characterization of the genomic stress responses of many organisms beyond model taxa. They are especially appropriate for organisms such as reef-building corals, for which dramatic declines in abundance are expected to worsen as anthropogenic climate change intensifies. Different corals differ substantially in physiological resilience to environmental stress, but the molecular mechanisms behind enhanced coral resilience remain unclear. Here, we compare transcriptome-wide gene expression (via RNA-Seq using Illumina sequencing) among conspecific thermally sensitive and thermally resilient corals to identify the molecular pathways contributing to coral resilience. Under simulated bleaching stress, sensitive and resilient corals change expression of hundreds of genes, but the resilient corals had higher expression under control conditions across 60 of these genes. These "frontloaded" transcripts were less up-regulated in resilient corals during heat stress and included thermal tolerance genes such as heat shock proteins and antioxidant enzymes, as well as a broad array of genes involved in apoptosis regulation, tumor suppression, innate immune response, and cell adhesion. We propose that constitutive frontloading enables an individual to maintain physiological resilience during frequently encountered environmental stress, an idea that has strong parallels in model systems such as yeast. Our study provides broad insight into the fundamental cellular processes responsible for enhanced stress tolerances that may enable some organisms to better persist into the future in an era of global climate change.