Platinum nanoparticle antioxidants inhibit pulmonary inflammation in mice exposed to cigarette smoke
Platinum nanoparticle antioxidants inhibit pulmonary inflammation in mice exposed to cigarette smoke
复制标题
DOI：
10.1016/j.pupt.2008.12.015
复制
发表时间：
2009-08-01
影响因子：
3.2
通讯作者：
Nagai, Atsushi
中科院分区：
文献类型：
--
作者：
Onizawa, Shigemitsu;Aoshiba, Kazutetsu;Nagai, Atsushi
最近的证据表明，增加的氧化应激是发生在吸烟者肺部炎症的重要机制。由于香烟烟雾（CS）含有并产生大量引起肺部炎症的活性氧（ROS），抗氧化剂可能成为与CS相关的炎症性肺部疾病（如慢性阻塞性肺疾病）的有效治疗剂。聚丙烯酸酯稳定的铂纳米颗粒形成稳定的胶体溶液（PAA-Pt）是一类新型抗氧化剂，已被证明可以有效地抑制活性氧。本研究探讨了PAA-Pt对吸烟小鼠肺部炎症的治疗作用。DBA/2小鼠经鼻给药PAA-Pt或生理盐水，然后每天暴露于CS或对照空气中3天。小鼠在最后暴露于CS或对照空气后4 h处死。CS暴露导致小鼠肺部抗氧化能力下降、NF κ B活化和中性粒细胞炎症，而在CS暴露前经鼻给药PAA-Pt可抑制这些变化。单独经鼻给药PAA-Pt不会引起肺部炎症或毒性。在体外实验中，用PAA-Pt处理肺泡ii型样A549细胞可抑制暴露于CS提取物后的细胞死亡。这些结果表明，铂纳米颗粒具有抗氧化剂的作用，可以抑制急性吸烟引起的肺部炎症。(C) 2008 Elsevier Ltd版权所有。
Recent evidence implicates increased oxidative stress as an important mechanism of the pulmonary inflammation that occurs in cigarette smokers. Since cigarette smoke (CS) contains and generates a large amount of reactive oxygen species (ROS) that elicit pulmonary inflammation, antioxidants may become effective therapeutic agents for CS-related inflammatory lung diseases, such as chronic obstructive pulmonary disease. Platinum nanoparticles stabilized with polyacrylate to form a stable colloid solution (PAA-Pt) are a new class of antioxidants that has been shown to efficiently quench ROS. In the present study we investigated the therapeutic effects of PAA-Pt on pulmonary inflammation in smoking mice. PAA-Pt or saline was administered intranasally to DBA/2 mice, which were then exposed to CS or control air daily for 3 days. Mice were sacrificed 4 h after their final exposure to CS or control air. CS exposure caused depletion of antioxidant capacity, NF kappa B activation, and neutrophilic inflammation in the lungs of mice, and intranasal administration of PAA-Pt prior to CS exposure was found to inhibit these changes. Intranasal administration of PAA-Pt alone did not elicit pulmonary inflammation or toxicity. In in vitro experiments, treatment of alveolar-type-II-like A549 cells with PAA-Pt inhibited cell death after exposure to a CS extract. These results suggest that platinum nanoparticles act as antioxidants that inhibit pulmonary inflammation induced by acute cigarette smoking. (C) 2008 Elsevier Ltd. All rights reserved.