Malaria vaccines: using models of immunity and functional genomics tools to accelerate the development of vaccines against Plasmodium falciparum.
Malaria vaccines: using models of immunity and functional genomics tools to accelerate the development of vaccines against Plasmodium falciparum.
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疟疾疫苗：利用免疫模型和功能基因组学工具加速恶性疟原虫疫苗的开发。
DOI：
10.1016/j.vaccine.2005.01.046
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Fried,Michal
中科院分区：
文献类型：
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作者：
Duffy,PatrickE;Krzych,Urszula;Francis,Susan;Fried,Michal
自然获得的免疫力和用减毒寄生虫免疫后获得的免疫力表明抗疟疾疫苗是可行的。一些障碍阻碍了疟疾疫苗的开发，其中包括我们对保护性免疫的了解不足，以及研究恶性疟原虫寄生虫的基因和蛋白质表达的技术困难。妊娠疟疾为疫苗开发提供了一种模型方法：最近的研究结果阐明了这种综合征的疾病发病机制和保护性免疫的基础，这种理解使人们集中精力确定妊娠疟疾疫苗的最佳抗原。与此同时，功能基因组学工具正在克服研究疟原虫蛋白质表达的几个障碍，大大加快了抗原发现的步伐。总之，这些概念和技术的进步使得疫苗抗原选择的合理方法成为可能，其中根据表达模式和特定特征从整个基因组中选择有限数量的抗原。然后根据通过了解发病机制和保护性免疫而建立的标准对这些候选抗原进行详细研究，以确定包含在亚单位疫苗中的最佳抗原。
Naturally acquired immunity and immunity acquired after immunization with attenuated parasites indicate that a vaccine against malaria is feasible. Several obstacles have stymied malaria vaccine development, among them our poor understanding of protective immunity and technical difficulties for studying gene and protein expression in the Plasmodium falciparum parasite. Pregnancy malaria offers a model approach for vaccine development: recent findings have elucidated the basis for disease pathogenesis and protective immunity in this syndrome, and this understanding has focused the effort to identify the optimal antigens for a pregnancy malaria vaccine. In parallel, functional genomics tools are overcoming several of the obstacles for studying protein expression in the malaria parasite, vastly accelerating the pace for antigen discovery. Together, these conceptual and technological advances allow a rational approach to vaccine antigen selection, in which a finite number of antigens are selected from the entire genome by merit of the expression patterns and specific features. These candidate antigens are then subjected to detailed studies according to criteria established by the understanding of pathogenesis and protective immunity, to identify the optimal antigens for inclusion in subunit vaccines.