Blocking pterygopalatine arterial blood flow decreases infarct volume variability in a mouse model of intraluminal suture middle cerebral artery occlusion
Blocking pterygopalatine arterial blood flow decreases infarct volume variability in a mouse model of intraluminal suture middle cerebral artery occlusion
复制标题
DOI：
10.1016/j.jneumeth.2008.06.021
复制
发表时间：
2008-09-15
影响因子：
3
通讯作者：
Saito, Nobuhito
中科院分区：
文献类型：
--
作者：
Chen, Yili;Ito, Akihiro;Saito, Nobuhito
大脑中动脉闭塞（MCAO）的小鼠模型仍然与几个问题有关，如梗死体积和存活率的可变性。因此，该方法远非标准化。在小鼠MCAO模型中翼腭动脉（PPA）中的血流的影响仍然不清楚。在使用市售的硅酮涂层单丝制备小鼠MCAO模型的同时，我们暂时闭塞颈总动脉（CCA）或PPA以确定脑血流量（CBF）值、梗死面积和模型的稳定性是否会受到影响。将40只雄性C57 BL/6小鼠分为3组：CCA血流阻断的MCAO（MCAO-C; n = 12）、PPA血流阻断的MCAO（MCAO-P; n = 16）和没有CCA或PPA血流阻断的MCAO（MCAO-U; n = 12）。我们发现MCAO-U组在闭塞期间的CBF值显著高于其他两组（p < 0.001）。在再灌注后24小时，我们用2%氯化2，3，5-三苯基四氮唑对每组的全脑进行染色。MCAO-U组与其他两组的平均梗死体积无明显差异，但MCAO-U组的梗死体积差异显著大于其他两组（p < 0.05）。我们的结论是，侧支循环从PPA到大脑显着影响MCAO模型，不能忽视。使用市售硅胶涂层缝线可生成大致一致的小鼠MCAO模型，同时在闭塞期间阻断PPA血流。(C)2008 Elsevier B. V.保留所有权利。
The mouse model of intraluminal suture middle cerebral artery occlusion (MCAO) is still associated with several issues, such as variability of infarction volume and survival. Thus, the method is far from standardization. The effect of blood flow in the pterygopalatine artery (PPA) in the mouse MCAO model remains obscure. While producing mouse MCAO models using commercially available silicone-coated monofilaments, we temporarily occluded the common carotid artery (CCA) or PPA to determine whether cerebral blood flow (CBF) values, infarct size and the stability of the model would be affected. Forty male C57BL/6 mice were divided into 3 groups: MCAO with blocked CCA blood flow (MCAO-C; n = 12), MCAO with blocked PPA blood flow (MCAO-P; n = 16) and MCAO without either CCA or PPA blood flow blockage (MCAO-U; n = 12). We found that the CBF values were significantly higher during occlusion in the MCAO-U than in the other two groups (p < 0.001). We stained whole brains from each group at 24 h after reperfusion with 2% 2,3,5-triphenyltetrazolium chloride. Although mean infarct volume did not obviously differ between the MCAO-U and other two groups, infarct volumes varied significantly more within the MCAO-U, than in the other two groups (p < 0.05). We concluded that collateral circulation from the PPA to the brain significantly influences the MCAO model, and cannot be ignored. An approximately consistent mouse MCAO model can be generated using commercially available silicone-coated sutures while blocking PPA blood flow during occlusion. (C) 2008 Elsevier B.V. All rights reserved.