Vinyl sulfone-based ferrocenylation reagents: applications in conjugation and bioconjugation
Vinyl sulfone-based ferrocenylation reagents: applications in conjugation and bioconjugation
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DOI：
10.1039/c3ob27209e
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发表时间：
2013-01-01
影响因子：
3.2
通讯作者：
Santoyo-Gonzalez, Francisco
中科院分区：
文献类型：
--
作者：
Megia-Fernandez, Alicia;Hernandez-Mateo, Fernando;Santoyo-Gonzalez, Francisco
二茂铁易于乙烯砜衍生化，可以制备一些有效、通用和有价值的二茂铁基化试剂。描述了这些化合物在温和条件下通过michael型加成对含有胺和/或硫醇的分子和生物分子进行偶联和生物偶联的适用性，从而保留后者的生物功能。通过制备多种偶联物和生物偶联物（端有二茂铁基的树状大分子和二茂铁-糖、二茂铁-环糊精、二茂铁-肽和二茂铁-蛋白质偶联物），证明了该方法的可行性。
The easy vinyl sulfone derivatization of ferrocene allows the preparation of some effective, versatile and valuable ferrocenylation reagents. The applicability of such compounds in conjugation and bioconjugation of amine and/or thiol containing molecules and biomolecules through Michael-type addition under mild conditions that preserve the biological function of the latter is described. The feasibility of the methodology is demonstrated by the preparation of a variety of conjugates and bioconjugates (ferrocenyl terminated dendrimers and ferrocene-sugar, ferrocene-cyclodextrin, ferrocene-peptide and ferrocene-protein conjugates).