Theoretical and experimental characterization of polyamine/DNA interactions.
Theoretical and experimental characterization of polyamine/DNA interactions.
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多胺/DNA 相互作用的理论和实验表征。
DOI：
10.1007/978-1-4684-5637-0_46
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发表时间：
1988
影响因子：
--
通讯作者：
Marton,LJ
中科院分区：
文献类型：
--
作者：
Feuerstein,BG;Basu,HS;Marton,LJ
多年来，我们实验室的工作一直致力于了解多胺和核酸的相互作用。这项研究的最初推动力是发现，在通过 α-二氟甲基鸟氨酸预处理降低了细胞内多胺水平的肿瘤细胞中，抗肿瘤药物（如 ascis-platinum 和 2-氯乙基亚硝基脲）的细胞毒性发生了改变 (1, 2)。据认为，这些药物通过形成 DNA 链间交联来杀死细胞，从而阻止复制并导致细胞死亡。多胺消耗对细胞毒性影响的一种可能解释是细胞内 DNA 的构象发生改变，这会影响交联形成的机制。
For several years, work in our laboratory has been directed toward an understanding of the interactions of polyamines and nucleic acids. The initial impetus to this research was the finding that the cytotoxicity of antineoplastic agents such ascis-platinum and the 2-chloroethylnitrosoureas are altered in tumor cells in which intracellular levels of polyamines had been depleted by pretreatment with α-difluoromethylornithine (1, 2). It is thought that these agents kill cells by forming DNA interstrand crosslinks that prevent replication and lead to cell death. One possible explanation for the effects of polyamine depletion on cytotoxicity is that the conformation of intracellular DNA is altered, which would affect the mechanism of crosslink formation.