A fluorescence-quenching method for quantitative analysis of Ponceau 4R in beverage.
A fluorescence-quenching method for quantitative analysis of Ponceau 4R in beverage.
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DOI：
10.1016/j.foodchem.2016.11.100
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发表时间：
2017-04
期刊：
影响因子：
8.8
通讯作者：
Jianpo Zhang;Lihua Na;Yunxia Jiang;Dandan Han;Dawei Lou;Li Jin
中科院分区：
文献类型：
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作者：
Jianpo Zhang;Lihua Na;Yunxia Jiang;Dandan Han;Dawei Lou;Li Jin
合成了CdTe量子点，并用其对溶液中的丽春红4R进行了定量分析。当激发波长为380 nm时，Ponceau 4R对CdTe量子点的发射有明显的猝灭作用。为了在温和的条件下检测Ponceau 4R，考察了CdTe量子点的荧光发射波长、pH值、温度和反应时间对检测结果的影响，建立了实验条件。利用量子点荧光强度对丽春红4R的线性响应，使丽春红4R的定量分析范围为2.5~2 5μg/mL，检出限为0.025μg/mL。此外，利用修正的Stern-Volmer方程和热力学计算，对该反应体系的反应机理进行了详细的研究。并用该方法对实际样品进行了定量分析，表明该方法可以在类似样品中得到更广泛的应用。
CdTe quantum dots was synthesized and used to quantitative analysis of Ponceau 4R in solution. With the excitation wavelength of 380 nm the emission of CdTe quantum dots was quenched obviously by Ponceau 4R. In order to detect Ponceau 4R in mild condition the influences of fluorescence emission wavelength of CdTe quantum dots, pH value, temperature and reaction time were examined to establish the experimental condition. The linear response of the fluorescence intensity of CdTe quantum dots to Ponceau 4R allowed the quantitative analysis of Ponceau 4R in a range of 2.5–25 μg/mL, and the limit of detection for Ponceau 4R was 0.025 μg/mL. In addition, the responsive mechanism of this reaction system was investigated in detail by using the modified Stern-Volmer equation and thermodynamic calculation. Particularly, this method was used to quantitatively analyze the real sample, which indicated that this method could be more widely applied in similar samples.