Improved Sensitivity in the Measurement of Residual Leukocytes in Platelet Products Using an Automated Leukocyte Counter
Improved Sensitivity in the Measurement of Residual Leukocytes in Platelet Products Using an Automated Leukocyte Counter
复制标题
使用自动白细胞计数器提高血小板产品中残留白细胞测量的灵敏度
作者：
H. Nagahashi;Katsumi Yabusaki;H. Matsui;T. Hirono;N. Yokokawa;Masahiro Satake;Kenji Tadokoro;T. Juji
Background and Objectives: Leukocytes remaining in platelet concentrates may be responsible for side effects caused by platelet transfusion. A simple method of high sensitivity for determining trace numbers of leukocytes is currently needed. Materials and Methods: An automated leukocyte counter, the LD-1000, the principle of which is a combination of spinning down on the observation field of stained nuclei derived from a 100 μl neat sample and image digitization with a charge coupled device camera, was newly developed and tested for sensitivity and reproducibility. Results: While the theoretical lower limit of detection was 0.01 cells/μl, the limit of detection of the LD-1000 was verified to be 0.2 cells/μl. A good correlation (r = 0.86) was observed between the results obtained using the Nageotte method and the LD-1000. Repeated measurements also confirmed satisfactory reproducibility. Conclusions: The instrument provides a new method of enumerating residual leukocytes in platelet products with better sensitivity and easier procedure compared to the Nageotte method and will be useful for quality assurance purposes at blood centers.
血小板减少白细胞和紫外线 B 照射，以防止同种免疫和血小板输注无效。
DOI：
10.1056/nejm199712253372601
发表时间：
1997
期刊：
The New England journal of medicine
影响因子：
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作者：
TrialtoReduceAlloimmunizationtoPlateletsStudyGroup
通讯作者：
TrialtoReduceAlloimmunizationtoPlateletsStudyGroup