Efficacy of antimicrobial polymer coatings in an animal model of bacterial infection associated with foreign body implants
Efficacy of antimicrobial polymer coatings in an animal model of bacterial infection associated with foreign body implants
复制标题
DOI：
10.1093/jac/dkq057
复制
发表时间：
2010-05-01
影响因子：
5.2
通讯作者：
Robins-Browne, Roy
中科院分区：
文献类型：
--
作者：
Hart, Emily;Azzopardi, Kristy;Robins-Browne, Roy
为了评估由聚对苯二甲酸乙二醇酯 (PET) 组成的支撑盘，其涂有不同的聚合物/左氧氟沙星组合，以在感染动物模型中发挥抗菌活性，从而探索使用包含左氧氟沙星的特定聚合物涂层作为减少器械相关感染的方法。制备了含有不同比例的聚乳酸二醇和聚己内酯二醇的脂肪族聚酯聚氨酯，与左氧氟沙星混合，然后用于涂覆支撑物光盘。在水溶液中测量这些盘的体外左氧氟沙星释放曲线。通过手术将带涂层的圆盘植入皮下，并通过将 10(6) cfu 金黄色葡萄球菌注射到包含植入物的皮下袋中来引发感染。 5、10、20和30天后，取出椎间盘，测定粘附在植入物上的细菌数量和椎间盘的残留抗菌活性。在体外，左氧氟沙星从涂层椎间盘中以恒定速率释放，然后在不同时间点达到平台，具体取决于所用的聚合物制剂。在体内，涂有含有左氧氟沙星的聚合物混合物的椎间盘均未被金黄色葡萄球菌定植，而仅涂有聚合物的椎间盘则有 94% 被感染。所有涂有左氧氟沙星混合聚合物的椎间盘在植入后至少 20 天都显示出残留的抗菌活性。含有左氧氟沙星的生物可蚀性聚酯聚氨酯聚合物涂层可以防止装置相关感染的术中模型中植入物的细菌定植。
To assess support discs, comprising polyethylene terephthalate (PET), coated with different polymer/levofloxacin combinations for antimicrobial activity in an animal model of infection, in order to explore the use of specific polymer coatings incorporating levofloxacin as a means of reducing device-related infections.Aliphatic polyester-polyurethanes containing different ratios of poly(lactic acid) diol and poly(caprolactone) diol were prepared, blended with levofloxacin and then used to coat support discs. The in vitro levofloxacin release profiles from these discs were measured in aqueous solution. Mice were surgically implanted with the coated discs placed subcutaneously and infection was initiated by injection of 10(6) cfu of Staphylococcus aureus into the subcutaneous pocket containing the implant. After 5, 10, 20 and 30 days, the discs were removed, and the number of bacteria adhering to the implant and the residual antimicrobial activity of the discs were determined.In vitro, the release of levofloxacin from the coated discs occurred at a constant rate and then reached a plateau at different timepoints, depending on the polymer preparation used. In vivo, none of the discs coated with polymer blends containing levofloxacin was colonized by S. aureus, whereas 94% of the discs coated with polymer alone were infected. All discs coated with levofloxacin-blended polymers displayed residual antimicrobial activity for at least 20 days post-implantation.Bioerodable polyester-polyurethane polymer coatings containing levofloxacin can prevent bacterial colonization of implants in an intra-operative model of device-related infections.