Cyclin D1 overexpression in non-Hodgkin's lymphoma with chromosome 11 bcl-1 rearrangement.
Cyclin D1 overexpression in non-Hodgkin's lymphoma with chromosome 11 bcl-1 rearrangement.
复制标题
细胞周期蛋白 D1 在具有 11 号染色体 bcl-1 重排的非霍奇金淋巴瘤中过度表达。
DOI：
10.1093/annonc/5.suppl_1.s71
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Swerdlow,SH
中科院分区：
文献类型：
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作者：
Williams,ME;Swerdlow,SH
大约70%的单核细胞淋巴瘤存在染色体易位t(11;14) （q13; q32）和相关的bcl-1断点位点和细胞周期蛋白Dl （PRAD1, CCND1）基因重排，从而暗示该基因参与了单核细胞淋巴瘤的发病机制。为了检测细胞周期蛋白D1在有或没有bcl-1或细胞周期蛋白D1重排的造血肿瘤中的表达，我们进行了northern blot分析。方法从白血病期造血肿瘤患者外周血细胞中分离总RNA，其中包括3例染色体11q13bcl-1重排的b细胞淋巴瘤患者。Northern blots与cyclin D1 cDNA探针杂交，测定mRNA表达程度。结果bcl-1重排病例的RNA均表达cyclin D1，而缺乏bcl-1或cyclin D1重排的b细胞CLL、t细胞原淋巴细胞白血病和急性非淋巴细胞白血病的RNA均未检测到表达。结论参与细胞周期调控的G1细胞周期蛋白cyclin D1的过表达可能在t（11; 14）阳性淋巴瘤的发病机制中起关键作用。
BackgroundApproximately 70% of centrocytic (mantlecell) lymphomas have the chromosomal translocation t(11;14) (ql3; q32) and associated rearrangements at thebcl-1 breakpoint locus and at the cyclin Dl (PRAD1, CCND1) gene, thus implicating this gene in the pathogenesis of centrocytic lymphoma. In order to determine cyclin D1 expression in hematopoietic neoplasms with and withoutbcl-1 or cyclin D1 rearrangements, northern blot analysis was performed.MethodsTotal RNA was isolated from peripheral blood cells of patients with hematopoietic neoplasms in leukemic phase, including three patients with B-cell lymphoma containing chromosome 11q13bcl-1 rearrangements. Northern blots were hybridized with a cyclin D1 cDNA probe and the degree of mRNA expression determined.ResultsEach of thebcl-1-rearranged cases showed high levels of cyclin D1 expression, whereas no expression was detected in RNA from samples of B-cell CLL, T-cell prolymphocytic leukemia, or acute nonlymphocytic leukemia which lackedbcl-1 or cyclin D1 rearrangement.ConclusionsOverexpression of cyclin D1, a G1 cyclin implicated in cell-cycle regulation, may play a critical role in the pathogenesis of t(11; 14)-positive lymphomas.