ScRNA-seq discover cell cluster change under OAB——ACE2 expression reveal possible alternation of 2019-nCoV infectious pathway
ScRNA-seq discover cell cluster change under OAB——ACE2 expression reveal possible alternation of 2019-nCoV infectious pathway
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DOI：
10.1101/2020.06.05.137380
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发表时间：
2020-06
期刊：
影响因子：
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通讯作者：
Bo Xiang;Xiangyu Hu;Haoxuan Li-;Li Ma;Hao Zhou;Lingling Wei;Jue Fan;Ji Zheng
中科院分区：
文献类型：
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作者：
Bo Xiang;Xiangyu Hu;Haoxuan Li-;Li Ma;Hao Zhou;Lingling Wei;Jue Fan;Ji Zheng
目的既往研究表明表达ACE 2的膀胱细胞是2019-nCov的潜在感染途径。本研究通过观察膀胱过度活动症（overactive bladder，OAB）小鼠与正常小鼠膀胱细胞簇及其ACE 2表达的差异，揭示了OAB环境下膀胱过度活动症（overactive bladder，OAB）感染可能性和途径的改变。材料与方法采用Pubic数据集获取人正常膀胱和小鼠膀胱（GSE 129845）中ACE 2的表达。我们建立了OAB模型，研究了OAB对细胞分型和ACE 2表达的影响。通过使用单细胞RNA测序（scRNA-seq）技术，分别测量膀胱细胞聚类和ACE 2在各种细胞类型中的表达。结果在公开数据库中（健康人和小鼠膀胱），ACE 2在人和小鼠膀胱中的表达集中在膀胱上皮细胞。在我们的OAB模型中，发现膀胱上皮的组成部分伞细胞消失。在两个小鼠膀胱样本中，上皮细胞ACE 2表达为34.1%，也是所有细胞类型中最高的。结论伞细胞的消失可能改变了2019-nCov的感染途径，并与OAB的发生和进展有关。
Objective Previous study indicated that bladder cells which express ACE2 were a potential infection route of 2019-nCov. This study observed some differences of bladder cell cluster and their ACE2 expression between OAB mice and healthy mice, indicating the change of infectious possibility and pathway under overactive bladder (OAB) circumstance. Material and method Pubic dataset acquisition was used to get ACE2 expression in normal human bladder and mice bladder (GSE129845). We built up over OAB model and studied the impact on cell typing and ACE2 expression. By way of using single-cell RNA sequencing (scRNA-seq) technique, bladder cell clustering and ACE2 expression in various cell types were measured respectively. Result In pubic database (healthy human and mice bladder), ACE2 expression in humans and mice is concentrated in bladder epithelial cells. The disappearance of umbrella cells, a component of bladder epithelial, was found in our OAB model. In the two mouse bladder samples, ACE2 expression of epithelial cells is 34.1%, also the highest of all cell types. Conclusion The disappearance of umbrella cell may alternate the infection pathway of 2019-nCov and relate to the onset and progression of OAB.