Three-Dimensional Objects Consisting of Hierarchically Assembled Nanofibers with Controlled Alignments for Regenerative Medicine
Three-Dimensional Objects Consisting of Hierarchically Assembled Nanofibers with Controlled Alignments for Regenerative Medicine
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DOI：
10.1021/acs.nanolett.9b00217
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发表时间：
2019-03-01
期刊：
影响因子：
10.8
通讯作者：
Xie, Jingwei
中科院分区：
文献类型：
--
作者：
Chen, Shixuan;Wang, Hongjun;Xie, Jingwei
对于再生医学来说，将具有可控取向的电纺纳米纤维组装成三维(3D)、复杂和预先设计的形状已被证明是一项艰巨的任务。在这里，我们报告了一种受旋转固体启发的新方法，该方法将厚度可控的二维(2D)纳米纤维垫转换为具有预先设计形状的曾经不可访问的3D对象。3D物体具有高度的渗透性，由排列的纳米纤维层组成，间距从几微米到几毫米不等。在压缩后，这些对象能够恢复其原始形状。多孔物体可以作为支架，引导细胞的组织并产生高度有序的3D组织结构。此外，对大鼠的皮下植入表明，3D对象能够快速穿透细胞、形成新血管和胶原基质沉积。这种新型的3D层次化纳米纤维结构在复杂3D组织结构/模型或器官的体外工程以及体内组织修复和再生方面都有很大的进展。
Assembling electrospun nanofibers with controlled alignment into three-dimensional (3D), complex, and predesigned shapes has proven to be a difficult task for regenerative medicine. Herein, we report a novel approach inspired by solids of revolution that transforms two-dimensional (2D) nanofiber mats of a controlled thickness into once-inaccessible 3D objects with predesigned shapes. The 3D objects are highly porous, consisting of layers of aligned nanofibers separated by gaps ranging from several micrometers to several millimeters. Upon compression, the objects are able to recover their original shapes. The porous objects can serve as scaffolds, guiding the organization of cells and producing highly ordered 3D tissue constructs. Additionally, subcutaneous implantation in rats demonstrates that the 3D objects enable rapid cell penetration, new blood vessel formation, and collagen matrix deposition. This new class of 3D hierarchical nanofiber architectures offers promising advancements in both in vitro engineering of complex 3D tissue constructs/models or organs and in vivo tissue repair and regeneration.