ACID-BASE EQUILIBRIA OF FLUORESCEIN AND 2',7'-DICHLOROFLUORESCEIN IN THEIR GROUND AND FLUORESCENT STATES
ACID-BASE EQUILIBRIA OF FLUORESCEIN AND 2',7'-DICHLOROFLUORESCEIN IN THEIR GROUND AND FLUORESCENT STATES
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DOI：
10.1021/j100672a011
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发表时间：
1971-01-01
影响因子：
--
通讯作者：
LIVINGSTON, R
中科院分区：
文献类型：
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作者：
LEONHARDT, H;GORDON, L;LIVINGSTON, R
酸性形式的二氯荧光素比相应形式的荧光素具有更强的酸性。阳离子和单阴离子的pk之差为1.7；中性酸的pk之差为0.9。荧光素和二氯荧光素的阴离子酸在它们的第一激发态中都略弱于它们的基态，pK之差为-0.2。测定了这两种染料在水溶液中的荧光光谱，范围从8MHCKh到pH为12。估算了双阴离子、单阴离子和阳离子形式的发射光谱。这些中碳酸似乎是非荧光的。
The acidic forms of dichlorofluorescein are stronger acids than the corresponding forms of fluorescein. For the cationic and monoanionic acids, the difference in pK is 1.7; for the neutral acids, it is 0.9. The anionic acids of fluorescein and dichlorofluorescein are both slightly weaker in their first excited singlet than in their ground states, the difference in pK being—0.2. The fluorescence spectra of both dyes were measured in aqueous solutions, ranging from 8 m HCKh to a pH of 12. The emission spectra of the dianonic, monoanonic, and cationic forms have been estimated. Theneutral acids appear to be nonfluorescent.