Exogenous N-acyl-homoserine lactones enhance the expression of flagella of Pseudomonas syringae and activate defence responses in plants
Exogenous N-acyl-homoserine lactones enhance the expression of flagella of Pseudomonas syringae and activate defence responses in plants
复制标题
外源N-酰基高丝氨酸内酯增强丁香假单胞菌鞭毛表达并激活植物防御反应
DOI：
10.1111/mpp.12502
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发表时间：
2018-01-01
影响因子：
4.9
通讯作者：
Fray, Rupert G.
中科院分区：
文献类型：
--
作者：
Cheng, Feifei;Ma, Anzhou;Fray, Rupert G.
为了科普病原体，植物已经进化出复杂的机制来感知病原体的攻击并诱导防御反应。N-酰基高丝氨酸内酯（阿勒）介导的细菌群体感应调节多种生理过程，包括致病性。在这项工作中，我们研究了AHL转基因烟草植物和假单胞菌pv。烟粉虱11528（P.与野生型植物相比，在AHL产生植物中观察到病害发病率降低和P. erichingae 11528生长减少。目前的数据表明，植物产生的AHLs增强对这种病原体的抗病性。随后的RNA测序分析表明，AHLs的外源添加上调了P. eriningae 11528基因的表达，用于鞭毛的产生。通过实时定量聚合酶链反应测定AHL生产和野生型植物中植物防御基因的表达水平。这些数据表明，植物产生的AHLs在接种后激活植物的广泛防御反应，包括氧化爆发，过敏反应，细胞壁强化和某些代谢产物的产生。这些结果表明，外源AHLs改变了病原菌的基因表达模式，植物产生的AHLs在植物感染的早期直接或间接地增强了植物的局部免疫。
In order to cope with pathogens, plants have evolved sophisticated mechanisms to sense pathogenic attacks and to induce defence responses. The N-acyl-homoserine lactone (AHL)-mediated quorum sensing in bacteria regulates diverse physiological processes, including those involved in pathogenicity. In this work, we study the interactions between AHL-producing transgenic tobacco plants and Pseudomonas syringae pv. tabaci 11528 (P. syringae 11528). Both a reduced incidence of disease and decrease in the growth of P. syringae 11528 were observed in AHL-producing plants compared with wild-type plants. The present data indicate that plant-produced AHLs enhance disease resistance against this pathogen. Subsequent RNA-sequencing analysis showed that the exogenous addition of AHLs up-regulated the expression of P. syringae 11528 genes for flagella production. Expression levels of plant defence genes in AHL-producing and wild-type plants were determined by quantitative real-time polymerase chain reaction. These data showed that plant-produced AHLs activated a wide spectrum of defence responses in plants following inoculation, including the oxidative burst, hypersensitive response, cell wall strengthening, and the production of certain metabolites. These results demonstrate that exogenous AHLs alter the gene expression patterns of pathogens, and plant-produced AHLs either directly or indirectly enhance plant local immunity during the early stage of plant infection.