NEUROMODULATORY EFFECTS OF CORTICOTROPIN RELEASING-FACTOR ON CEREBELLAR PURKINJE-CELLS - AN INVIVO STUDY IN THE CAT
NEUROMODULATORY EFFECTS OF CORTICOTROPIN RELEASING-FACTOR ON CEREBELLAR PURKINJE-CELLS - AN INVIVO STUDY IN THE CAT
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DOI：
10.1016/0306-4522(90)90238-y
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
BISHOP, GA
中科院分区：
文献类型：
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作者：
BISHOP, GA
促肾上腺皮质激素释放因子是一种由41个氨基酸组成的多肽，定位于猫S小脑皮质的爬行纤维和苔藓纤维。本研究将促肾上腺皮质激素释放因子应用于细胞外分离的浦肯野细胞，以观察促肾上腺皮质激素释放因子对神经元放电的影响。促肾上腺皮质激素释放因子本身对细胞活性几乎没有影响。然而，这种多肽增强了天冬氨酸和谷氨酸的兴奋作用，天冬氨酸和谷氨酸分别是攀援纤维和苔藓纤维平行纤维系统的神经递质。此外，促肾上腺皮质激素释放因子可阻断GABA离子导入的抑制作用。最后，它缩短或消除了小脑皮质中攀升纤维的激活所产生的抑制时间。这些数据表明，促肾上腺皮质激素释放因子在小脑回路中起神经调节剂而不是神经递质的作用。
Corticotropin releasing factor, a 41 amino acid peptide, has been localized in climbing fibers and mossy fibers in the cat''s cerebellar cortex. In the present study, corticotropin releasing factor was iontophoretically applied to Purkinje cells, isolated extracellulary, to assess the effect of this peptide on the firing rate of the neuron. By itself corticotropin releasing factor had little or no effect on cellular activity. However, this peptide potentiated the excitatory effects of aspartate and glutamate, the putative neurotransmitters of the climbing fiber and mossy fiber-parallel fiber systems, respectively. In addition, corticotropin releasing factor blocked the suppressive effects induced by the iontophoretic application of GABA. Finally, it shortened or eliminated the period of suppression produced by activation of climbing fibers in the cerebellar cortex. These data suggest that corticotropin releasing factor functions as a neuromodulator rather than as a neurotransmitter in cerebellar circuitry.