High resolution imaging using scanning ion conductance microscopy with improved distance feedback control
High resolution imaging using scanning ion conductance microscopy with improved distance feedback control
复制标题
DOI：
10.1016/j.pnsc.2008.01.011
复制
发表时间：
2008-06-10
影响因子：
4.7
通讯作者：
Klenerman, David
中科院分区：
文献类型：
--
作者：
Li, Chao;Johnson, Nicholas;Klenerman, David
显微镜是观察活细胞的基本技术。目前有很大的兴趣，在应用扫描探针显微镜图像生活在自然环境中的生物细胞在纳米尺度。扫描离子电导显微镜是一种新型的扫描探针显微镜，它能够对活生物细胞进行非接触式高分辨率成像。基于在生理缓冲液中扫描的纳米移液管，距离反馈控制使用离子电流来控制移液管尖端与样品表面之间的距离。然而，这种反馈控制在卷积细胞表面上的斜率上有困难，这限制了其分辨率。在这项研究中，我们提出了一种改进形式的反馈控制，从离子电流信号中去除了高达三阶斜率的贡献，从而提供了一个更准确的信号来控制距离。我们表明，这允许更快，更低的噪声地形高分辨率成像。(C)2008年国家自然科学基金、中国科学院。由Elsevier Limited和Science in China Press出版。All rights reserved.
Microscopy is an essential technique for observation on living cells. There is currently great interest in applying scanning probe microscopy to image-living biological cells in their natural environment at the nanometer scale. Scanning ion conductance microscopy is a new form of scanning probe microscopy, which enables non-contact high-resolution imaging of living biological cells. Based on a scanned nanopipette in physiological buffer, the distance feedback control uses the ion current to control the distance between the pipette tip and the sample surface. However, this feedback control has difficulties over slopes on convoluted cell surfaces, which limits its resolution. In this study, we present an improved form of feedback control that removes the contribution of up to the third-order slope from the ion current signal, hence providing a more accurate signal for controlling the distance. We show that this allows faster and lower noise topographic high-resolution imaging. (C) 2008 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.