Ectopic expression of Arabidopsis broad-spectrum resistance gene RPW8.2 improves the resistance to powdery mildew in grapevine (Vitis vinifera)
Ectopic expression of Arabidopsis broad-spectrum resistance gene RPW8.2 improves the resistance to powdery mildew in grapevine (Vitis vinifera)
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拟南芥广谱抗性基因RPW8.2的异位表达提高了葡萄（Vitis vinifera）对白粉病的抗性
DOI：
10.1016/j.plantsci.2017.11.005
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发表时间：
2018-02-01
期刊：
影响因子：
5.2
通讯作者：
Wen, Ying-Qiang
中科院分区：
文献类型：
--
作者：
Hu, Yang;Li, Yajuan;Wen, Ying-Qiang
白粉病是全世界栽培葡萄树最重要的经济病害。在此，我们报道拟南芥广谱抗病基因RPW8.2可以提高葡萄品种的白粉病抗性。汤普森无核。 RPW8.2-YFP 融合基因在葡萄树中通过 RPW8.2 的组成型 35S 启动子或天然启动子 (NP) 稳定表达。在没有任何病原体的情况下，35S::RPW8.2-YFP 转基因葡萄枝和小植株在发育后期显示出生根减少和生长减少。使用适应的葡萄白粉病分离株En NAFU1进行的感染测试表明，在表达两种构建体中的任一种的转基因葡萄树中，菌丝生长和孢子形成受到显着限制。这种抗性似乎可归因于 RPW8.2 的异位表达，并与吸器复合体 (EHC) 的包裹增强和 H2O2 的现场积累有关。此外，RPW8.2-YFP 融合蛋白在真菌渗透位点周围显示出聚集性聚集。转录组分析表明，葡萄藤中 RPW8.2 的异位表达不仅显着增强了水杨酸依赖性防御信号，而且还改变了其他植物激素相关基因的表达。综上所述，我们的结果表明 RPW8.2 可以用作转基因来提高葡萄树对白粉病的抗性。
Powdery mildew is the most economically important disease of cultivated grapevines worldwide. Here, we report that the Arabidopsis broad-spectrum disease resistance gene RPW8.2 could improve resistance to powdery mildew in Vitis vinifera cv. Thompson Seedless. The RPW8.2-YFP fusion gene was stably expressed in grapevines from either the constitutive 35S promoter or the native promoter (NP) of RPW8.2. The grapevine shoots and plantlets transgenic for 35S::RPW8.2-YFP showed reduced rooting and reduced growth at later development stages in the absence of any pathogens. Infection tests with an adapted grapevine powdery mildew isolate En NAFU1 showed that hyphal growth and sporulation were significantly restricted in transgenic grapevines expressing either of the two constructs. The resistance appeared to be attributable to the ectopic expression of RPW8.2, and associated with the enhanced encasement of the haustorial complex (EHC) and onsite accumulation of H2O2. In addition, the RPW8.2-YFP fusion protein showed focal accumulation around the fungal penetration sites. Transcriptome analysis revealed that ectopic expression of RPW8.2 in grapevines not only significantly enhanced salicylic acid-dependent defense signaling, but also altered expression of other phytohormofie-associated genes. Taken together, our results indicate that RPW8.2 could be utilized as a trans gene for improving resistance against powdery mildew in grapevines.