DETECTION OF COLLAGENASE ACTIVITY IN ORAL BACTERIA
DETECTION OF COLLAGENASE ACTIVITY IN ORAL BACTERIA
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DOI：
10.1139/m85-026
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发表时间：
1985-01-01
影响因子：
2.8
通讯作者：
GRENIER, D
中科院分区：
文献类型：
--
作者：
MAYRAND, D;GRENIER, D
12种菌的胶原溶解活性。使用2种检测方法评估口腔细菌。除2种外，所有测试的细菌菌株都能够降解至少一种一般蛋白质底物。生长测定中的胶原蛋白溶解活性的结果表明，当使用环氧乙烷对基质进行灭菌时，牙龈拟杆菌是唯一能够降解胶原蛋白的细菌。如果基质通过高压灭菌器灭菌，在存在或不存在生长培养基的情况下，其他细菌物种可显示为胶原溶解性的。当在[14C]胶原测定中使用完整或破碎的细胞时，也证明了胶原溶解活性。该测定和抑制研究的结果表明，胶原蛋白溶解活性可能是特异性胶原酶和非特异性蛋白酶的组合活性的结果（B. gingivalis）或仅使用非特异性蛋白酶（本研究中的其他菌株），尽管在后一种情况下，水解胶原蛋白所需的时间可能比使用特异性胶原酶长10倍。
Collagenolytic activity of 12 spp. of oral bacteria was assessed using 2 methods of detection. Except for 2 spp., all bacterial strains tested were capable of degrading at least 1 general protein substrate. Results of collagenolytic activity in a growth assay indicate that Bacteroides gingivalis is the only bacterium capable of degrading collagen when the substrate is sterilized using ethylene oxide. If the substrate is sterilized by autoclavine, in the presence or absence of the growth medium, other bacterial species could be shown to be collagenolytic. Collagenolytic activity was also demonstrated when whole or broken cells were used in a [14C]collagen assay. Results from this assay and from inhibition studies indicate that collagenolytic activity can be the result of the combined activities of both a specific collagenase and nonspecific proteases (B. gingivalis) or nonspecific proteases only (other strains in this study), although in the latter case, the time taken to hydrolyze collagen can be 10 times longer than with a specific collagenase.