Flow Cytometric Analysis of CD123 is Useful for Immunophenotyping Classical Hodgkin Lymphoma
Flow Cytometric Analysis of CD123 is Useful for Immunophenotyping Classical Hodgkin Lymphoma
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DOI：
10.1002/cyto.b.20561
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发表时间：
2011-03-01
影响因子：
3.4
通讯作者：
Fromm, Jonathan R.
中科院分区：
文献类型：
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作者：
Fromm, Jonathan R.
虽然经典霍奇金淋巴瘤（CHL）的诊断传统上是在组织切片中进行形态学检查，但现在可以通过流式细胞术（FC）诊断CHL。为了鉴定霍奇金和瑞德-斯特伯格（HRS）细胞上可能对FC免疫分型淋巴瘤有用的其他抗原，我们检测了HRS细胞上CD123 （IL-3R α链）的表达，并将其与其他淋巴结样本（372个组织样本，包括98个反应性病例和274个肿瘤病例）中CD123的表达进行了比较，使用九色FC试剂组合包括抗CD123。CD123在大多数HRS细胞群中表达（59例CHL淋巴结中59%），很少在b细胞非霍奇金淋巴瘤（NHLs）中表达（3例毛细胞白血病中有3例，29例慢性淋巴细胞白血病/小淋巴细胞淋巴瘤（CLL/SLL）中有3例，55例大b细胞淋巴瘤中有1例，但在95例其他b细胞NHL中没有表达），16例t细胞NHL中没有表达。在反应性淋巴结中，CD123的表达主要局限于浆细胞样树突状细胞和罕见的组织细胞群，这些细胞群很容易与HRS群体区分开来。由于该抗原在B-和T-NHL以及反应性淋巴结群体中的表达相对有限，因此评估CD123的表达有助于支持CHL的FC诊断。(C) 2010年国际临床细胞术学会
Although the diagnosis of classical Hodgkin lymphoma (CHL) is traditionally made morphologically in tissue sections, it is now possible to diagnose CHL by flow cytometry (FC). In an effort to identify additional antigens on Hodgkin and Reed-Sternberg (HRS) cells that might be useful for immunophenotyping this lymphoma by FC, we examined the expression of CD123 (alpha chain of the IL-3R) on HRS cells and compared this with the expression of CD123 in other lymph node samples (372 tissue specimens, including 98 reactive and 274 neoplastic cases), using a nine-color FC reagent combination including anti-CD123. CD123 was found to be expressed on the majority of HRS cell populations (59% of 59 CHL lymph nodes examined), rarely on B-cell non-Hodgkin lymphomas (NHLs) (3 of 3 hairy cell leukemia cases, 3 of 29 chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) cases, 1 of 55 large B-cell lymphoma cases, but not on 95 other B-cell NHL examined), and in none of the 16 T-cell NHL cases examined. In reactive lymph nodes, CD123 expression was largely limited to plasmacytoid dendritic cells and rare histiocyte populations that can readily be distinguished from HRS populations. As the expression of this antigen is shown to be relatively limited in B- and T-NHL and in reactive lymph node populations, assessment of CD123 expression is useful for supporting the FC diagnosis of CHL. (C) 2010 International Clinical Cytometry Society