Genetic variation in tomato populations from four breeding programs revealed by single nucleotide polymorphism and simple sequence repeat markers
Genetic variation in tomato populations from four breeding programs revealed by single nucleotide polymorphism and simple sequence repeat markers
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单核苷酸多态性和简单序列重复标记揭示了四个育种计划中番茄群体的遗传变异
DOI：
10.1016/j.scienta.2009.03.025
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发表时间：
2009-09-01
影响因子：
4.3
通讯作者：
Yang, Wencai
中科院分区：
文献类型：
--
作者：
Chen, Jia;Wang, Hui;Yang, Wencai
现代作物的遗传变异性由于驯化和育种而受到限制。以216个番茄（Solanum lycopersicum L.）利用单核苷酸多态性和简单重复序列标记对来自四个育种计划的栽培品种、杂交种和优良育种系进行基因分型。在所分析的47个标记中，72.3%的标记在216个基因型中表现出多态性，51.06-59.57%的标记在群体中表现出多态性。然而，在所有四个群体的遗传变异是狭窄的。群体间的Nei遗传距离为0.0422 ~ 0.1135，个体群体内品系间的Nei遗传距离为0.0085 ~ 0.3187。聚类分析和主坐标分析结果表明，4个种群可分为3个分支。来自沈阳农业大学和中国农业大学群体的品系构成第一分支，来自北京蔬菜研究中心的品系构成第二分支，来自Nunhems的品系构成第三分支。利用STRUCTURE2.2进行的群体结构分析进一步证实了这一点，并表明育种程序之间可能存在种质交换不足的问题。这可能是近年来我国园艺性状显著改良的新品种选育进展缓慢的原因。(C)2009 Elsevier B. V.保留所有权利。
Genetic variability in modern crops is limited due to domestication and breeding. To investigate genetic variation in different populations, 216 tomato (Solanum lycopersicum L.) cultivars, hybrids, and elite breeding lines from four breeding programs were genotyped using single nucleotide polymorphism and simple sequence repeat markers. Of 47 markers analyzed, 72.3% were polymorphic in the whole collection of 216 genotypes and 51.06-59.57% showed polymorphisms in individual populations. However, genetic variation was narrow in all four populations. Nei's genetic distance varied from 0.0422 to 0.1135 between populations and from 0.0085 to 0.3187 between lines in individual populations. Cluster and principal coordinate analysis indicated that the four populations could be grouped into three clades. Lines from Shenyang Agricultural University and China Agricultural University population formed the first clade, lines from Beijing Vegetable Research Center were in the second clade, and lines from Nunhems were in the third clade. This was further Supported by population structure analysis using STRUCTURE2.2, and suggested that a lack of germplasm exchange might exist among breeding programs. It might be the reason that the progress of developing new varieties with significant improvement of horticultural traits in China is slow in recent years. (C) 2009 Elsevier B.V. All rights reserved.