A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubles at the onset of cytokinesis
A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubles at the onset of cytokinesis
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DOI：
10.1016/s1534-5807(02)00402-1
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发表时间：
2003-01-01
影响因子：
11.8
通讯作者：
Saint, R
中科院分区：
文献类型：
--
作者：
Somers, WG;Saint, R
细胞因子收缩环的定位机制尚不清楚，但源自纺锤体中部区域。我们发现，Rho GTP 交换因子 Pebble 和 Rho 家族 GTP 酶激活蛋白 RacGAP50C 之间的相互作用，将收缩环与黑腹果蝇细胞中皱纹部位的皮质微管连接起来。 Pebble 调节肌动球蛋白组织，而 RacGAP50C 及其结合伙伴帕瓦罗蒂驱动蛋白样蛋白则调节微管成束。所有三个因素都是胞质分裂所必需的。当犁沟开始时，这些蛋白质共定位于皮质赤道环。我们提出 RacGAP50C-Pavarotti 复合物沿着皮质微管移动到细胞赤道，在那里它们与 Pebble RhoGEF 结合以定位收缩环的形成并协调胞质分裂期间的 F-肌动蛋白和微管重塑。
The mechanism that positions the cytokinetic contractile ring is unknown, but derives from the spindle mid-zone. We show that an interaction between the Rho GTP exchange factor, Pebble, and the Rho family GTPase-activating protein, RacGAP50C, connects the contractile ring to cortical microtubules at the site of furrowing in D. melanogaster cells. Pebble regulates actomyosin organization, while RacGAP50C and its binding partner, the Pavarotti kinesin-like protein, regulate microtubule bundling. All three factors are required for cytokinesis. As furrowing begins, these proteins colocalize to a cortical equatorial ring. We propose that RacGAP50C-Pavarotti complexes travel on cortical microtubules to the cell equator, where they associate with the Pebble RhoGEF to position contractile ring formation and coordinate F-actin and microtubule remodeling during cytokinesis.