Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.
Expression and characterization of vanilloid receptor subtype 1 in human dental pulp cell cultures.
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DOI：
10.1097/01.don.0000155259.22746.ae
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发表时间：
2005-09
影响因子：
4.2
通讯作者：
R. Miyamoto;M. Tokuda;T. Sakuta;S. Nagaoka;M. Torii
中科院分区：
文献类型：
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作者：
R. Miyamoto;M. Tokuda;T. Sakuta;S. Nagaoka;M. Torii
采用RT-PCR、Western blotting和免疫细胞化学方法检测人牙髓成纤维细胞（PF-10）中香草酸受体亚型1（VR 1、TRPV 1）的表达。ELISA结果显示，辣椒素可诱导PF-10细胞表达IL-6，VR 1拮抗剂capsazepine可剂量依赖性地抑制辣椒素诱导的IL-6表达，表明辣椒素诱导的IL-6表达与VR 1激活有关。研究了辣椒素与丝裂原活化蛋白激酶（MAPKs）的相互作用。辣椒素刺激后，检测p38 MAPK和c-Jun氨基末端激酶（JNK）的磷酸化。p38 MAPK参与辣椒素诱导的IL-6产生，如该激酶的特异性抑制剂的使用所示。EMSA结果显示辣椒素抑制肿瘤坏死因子-α（TNF-α）诱导的PF-10细胞核因子-κ B（NF-κ B）活化。这些结果表明，VR 1的激活在牙髓炎症中起重要作用。
The expression of the vanilloid receptor subtype 1 (VR1, TRPV1) was detected in human dental pulp fibroblasts (PF-10) using RT-PCR, Western blotting, and immunocytochemical analysis. As revealed by ELISA, capsaicin induced IL-6 expression in PF-10 cells, and the VR1 antagonist capsazepine dose-dependently inhibited capsaicin-induced IL-6 production, indicating that capsaicin-induced IL-6 expression is related to VR1 activation. The interaction between capsaicin and mitogen-activated protein kinases (MAPKs) was investigated. The phosphorylation of p38 MAPK and c-Jun NH2-terminal kinase (JNK) were detected after capsaicin stimulation. p38 MAPK is involved in capsaicin-induced IL-6 production, as shown by the use of specific inhibitors of this kinase. The result of EMSA showed that capsaicin inhibited tumor necrosis factor-alpha (TNF-alpha)-induced nuclear factor-kappa B (NF-kappaB) activation in PF-10 cell cultures. These results suggest that the activation of VR1 plays an important role in dental pulp inflammation.