Cloning and functional analysis of the human IRF-3 promoter
Cloning and functional analysis of the human IRF-3 promoter
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DOI：
10.1089/104454999314962
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发表时间：
1999-09-01
影响因子：
3.1
通讯作者：
Pitha, PM
中科院分区：
文献类型：
--
作者：
Lowther, WJ;Moore, PA;Pitha, PM
我们已经分离出人类IRF-3基因的基因组克隆，其包含5'侧翼区的779个核苷酸和完整的内含子外显子序列。该基因有八个外显子，在染色体 19q13.3 上跨度约 6 kb。 IRF-3启动子既没有保守的TATA盒，也没有CCAAT盒基序，但富含GC。鉴定了几个假定的 DNA 结合元件，包括三个 SP-1 位点、一个 USF 元件、一个 HOX 盒、一个 CarG 盒和一个 NF-1 位点。启动子区域的删除分析表明，核心基础启动子由第一个转录起始位点 5' 的 113 bp 组成，足以进行组成型表达。该区域仅包含 SP-1 位点之一以及 HOX 元件和 NF-1 位点，尽管它富含 GC，但它不包含任何其他假定的 DNA 结合位点。相反，小鼠 IRF-3 启动子虽然与人类启动子表现出高度同源性，但同时包含 TATA 和 CCAAT 盒基序，这表明至少在转录起始水平上，这些基因可能受到差异性调控。
We have isolated a genomic clone of the human IRF-3 gene containing 779 nucleotides of the 5' flanking region and the complete intron exon sequence. The gene has eight exons which span about 6 kb on chromosome 19q13.3. The IRF-3 promoter has neither a conserved TATA box nor a CCAAT box motif but is GC rich. Several putative DNA-binding elements were identified, including three SP-1 sites, a USF element, a HOX box, a CarG box, and an NF-1 site. Deletion analysis of the promoter region showed that the core basal promoter, consisting of 113 bp 5' of the first transcription start site, was sufficient for constitutive expression. This region contains only one of the SP-1 sites as well as the HOX element and NF-1 site, and although it is GC rich, it does not contain any of the other putative DNA-binding sites. Tn contrast, the mouse IRF-3 promoter, while displaying a high degree of homology with the human promoter, contains both TATA and CCAAT box motifs, suggesting that, at least at the level of transcription initiation, these genes may be differentially regulated.