Synthetic gene networks in mammalian cells.
Synthetic gene networks in mammalian cells.
复制标题
哺乳动物细胞中的合成基因网络。
DOI：
10.1016/j.copbio.2010.07.006
复制
发表时间：
2010
影响因子：
7.7
通讯作者：
M. Fussenegger
中科院分区：
文献类型：
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作者：
W. Weber;M. Fussenegger
具有复杂时空动力学的合成基因电路的设计和构建是在细菌中开创的，但合成生物学家花了将近十年的时间才能够在哺乳动物细胞中构建具有复杂时空动力学的遗传电路。这篇综述强调了哺乳动物合成生物学的最新进展，它描述了代谢物，激素和光触发的基因开关以及设计和构建具有可调振荡的合成网络。最后，我们讨论的不仅是目前的局限性，但也可能的方式来改造合成哺乳动物系统的建设从一门艺术到预测工程学科。
The design and construction of synthetic gene circuits with complex spatiotemporal dynamics was pioneered in bacteria, but it took almost a decade until synthetic biologists were able to construct genetic circuits with complex spatiotemporal dynamics in mammalian cells. This review highlights the most recent advances in mammalian synthetic biology, and it describes metabolite, hormone, and light-triggered genetic switches as well as the design and construction of synthetic networks that feature tunable oscillations. We conclude by discussing not only the current limitations but also possible ways to transform the construction of synthetic mammalian systems from an art into a predictive engineering discipline.
影响因子：
64.8
作者：
通讯作者：
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