Genetic Variation of Rice Blast (Pyricularia oryzae) Isolates in Laos
Genetic Variation of Rice Blast (Pyricularia oryzae) Isolates in Laos
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老挝稻瘟病菌的遗传变异
DOI：
10.1094/php-05-20-0041-rs
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发表时间：
2020
影响因子：
2.3
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Xangsayasane;P.;Boualaphanh;C.;Bounphanousay;C.;Bounphanousay;V.;Manivong;P.;Voradeth;S.;Inthapanya;P.;Sato;T.;Obara;M.;Hayashi;N.;Fukuta;Y.
2007年至2009年从老挝全境采集的192株稻瘟病菌的致病性表现出对差异品种（DV）和感病对照品种丽江新团黑谷的毒力频率差异很大。 Pit、Pia、Pi3、Pi5(t)、Pik-s、Piz-t、Pi19(t) 和 Pi20(t) 的 DV 反应中发现高毒力频率 (>50%)；在 Pib、Pii、Pik-m、Pi1、Pik-h、Pik、Pik-p、Pi7(t)、Piz-5、Pita（两条线）、Pita-2（两条线）和 Pi12(t) 的 DV 中发现了中间值（从 10 到 50%）；在 Pish、Piz 和 Pi9(t) 的 DV 中发现了低频 (<10%)。根据对稻瘟菌的反应模式，将稻瘟菌分为三类：Ia 和 Ib（低毒力）和 II（高毒力）。 Ia群中的稻瘟菌在雨养低地地区占优势，Ib群中的稻瘟菌在灌溉低地地区中占优势，II群中的稻瘟菌在旱地地区中占优势。 Ia、Ib 和 II 簇群分别在南部、中部和北部地区占主导地位。老挝稻瘟病小种根据水稻种植生态系统和地理区域从南到北分布，毒力不同。这些菌株被分为156个小种，每个小种中稻瘟菌的数量都很少。从中筛选出15个有代表性的菌株作为标准鉴别稻瘟菌株，建立鉴别系统。
The pathogenicity of 192 blast isolates collected from regions across the whole of Laos from 2007 to 2009 showed a wide variation in terms of the frequencies of virulence toward differential varieties (DVs) and the susceptible control cultivar Lijiangxintuanheigu. High frequencies of virulence (>50%) were found in the reactions of DVs forPit,Pia,Pi3,Pi5(t),Pik-s,Piz-t,Pi19(t), andPi20(t); intermediate values (from 10 to 50%) were found in DVs forPib,Pii,Pik-m,Pi1,Pik-h,Pik,Pik-p,Pi7(t),Piz-5,Pita(two lines),Pita-2(two lines), andPi12(t); and low frequencies (<10%) were found in DVs forPish,Piz, andPi9(t). The blast isolates were classified into three cluster groups: Ia and Ib (low virulence) and II (high virulence), based on the patterns of reaction to them. The blast isolates in cluster group Ia were dominant in rainfed lowland areas, those in Ib were dominant in irrigated lowland areas, and those in II were dominant in upland areas. Cluster groups Ia, Ib, and II were dominant in the Southern, Central, and Northern regions, respectively. Blast races in Laos were distributed according to ecosystems for rice cultivation and geographical regions from south to north with different virulence. These isolates were categorized into 156 races, and the numbers of blast isolates were few in each race. A total of 15 representative isolates were selected from among them as standard differential blast isolates, to develop a differential system.