Bioreactor performance and functional gene analysis of microbial community in a limited-oxygen fed bioreactor for co-reduction of sulfate and nitrate with high organic input.
Bioreactor performance and functional gene analysis of microbial community in a limited-oxygen fed bioreactor for co-reduction of sulfate and nitrate with high organic input.
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DOI：
10.1016/j.jhazmat.2014.06.006
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发表时间：
2014-08
影响因子：
13.6
通讯作者：
Xi-jun Xu;Chuan Chen;Ai-Jie Wang;Hao Yu;Xuefei Zhou;Hong-liang Guo;Ye Yuan;Duu-Jong Lee;Jizhong Zhou;N. Ren
中科院分区：
文献类型：
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作者：
Xi-jun Xu;Chuan Chen;Ai-Jie Wang;Hao Yu;Xuefei Zhou;Hong-liang Guo;Ye Yuan;Duu-Jong Lee;Jizhong Zhou;N. Ren
预测了硫酸盐还原细菌(SRB)、硝酸盐还原细菌(NRB)和硫化物氧化细菌(SOB，包括硝酸盐还原细菌、硫化物氧化细菌NR-SOB)之间的有限氧气介导的协同关系，以同时去除硝酸盐、硫酸盐和高COD污染物，并消除硫化物的产生。进行了实验室规模的实验，以考察有限的氧气对这些氧阴离子降解、硫化物氧化和相关微生物功能反应的影响。在测试的所有情况下，硝酸盐和硫酸盐的还原几乎都完成了。在生物反应器中充氧时，SO2的生成率显著提高，∼可达70%。利用功能基因芯片GeoChip 4.0分析了微生物在硝酸盐和硫酸盐还原以及硫化物氧化过程中的基因多样性和功能潜力。生物反应器中微生物群落的多样性随着投加氧气的增加而增加。而参与硫酸盐还原的功能基因的强度没有明显的差异，但在有限的氧气样本中，检测到的反硝化基因的丰度降低了。更重要的是，硫化物氧化细菌可能会改变其种群/基因，以响应有限的氧气，潜在地在硫化物氧化，特别是对元素硫的氧化中更有效地发挥作用。硝酸盐还原硫化物氧化细菌(NR-SOB)的fccA/fccB基因在低氧条件下更为丰富，如反硝化副球藻、脱氮硫杆菌、Beggiatoasp.、硫杆菌和硫代碱性弧菌等。
Limited-oxygen mediated synergistic relationships between sulfate-reducing bacteria (SRB), nitrate-reducing bacteria (NRB) and sulfide-oxidizing bacteria (SOB, including nitrate-reducing, sulfide-oxidizing bacteria NR-SOB) were predicted to simultaneously remove contaminants of nitrate, sulfate and high COD, and eliminate sulfide generation. A lab-scale experiment was conducted to examine the impact of limited oxygen on these oxy-anions degradation, sulfide oxidation and associated microbial functional responses. In all scenarios tested, the reduction of both nitrate and sulfate was almost complete. When limited-oxygen was fed into bioreactors, S0formation was significantly improved up to ∼70%. GeoChip 4.0, a functional gene microarray, was used to determine the microbial gene diversity and functional potential for nitrate and sulfate reduction, and sulfide oxidation. The diversity of the microbial community in bioreactors was increased with the feeding of limited oxygen. Whereas the intensities of the functional genes involved in sulfate reduction did not show a significant difference, the abundance of the detected denitrification genes decreased in limited oxygen samples. More importantly, sulfide-oxidizing bacteria may alter their populations/genes in response to limited oxygen potentially to function more effectively in sulfide oxidation, especially to elemental sulfur. The genes fccA/fccB from nitrate-reducing, sulfide-oxidizing bacteria (NR-SOB), such asParacoccus denitrificans,Thiobacillus denitrificans,Beggiatoasp.,Thiomicrospirasp., andThioalkalivibrio sp., were more abundant under limited-oxygen condition.