Cytoplasmic dynein and early endosome transport.
Cytoplasmic dynein and early endosome transport.
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DOI：
10.1007/s00018-015-1926-y
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Xiang X;Qiu R;Yao X;Arst HN Jr;Peñalva MA;Zhang J
基于微管的细胞器/囊泡的分布对于许多类型的真核细胞的功能是至关重要的，并且分子马达细胞质动力蛋白是向微管负端运输各种细胞货物所必需的。在丝状真菌中，早期内体是动力蛋白的主要载体，而动力蛋白调节因子如LIS 1和动力蛋白复合物都是早期内体运动所必需的。在真菌菌丝中，驱动蛋白3和动力蛋白驱动早期内体的双向运动。动力蛋白在微管正端积累;这种积累依赖于驱动蛋白-1和动力蛋白，并且对于早期内体朝向微管负端的运动是重要的。动力蛋白和早期内体之间的物理相互作用需要动力蛋白复合物，特别是其p25组分。FTS-钩-FHIP（FHF）复合物将动力蛋白-动力肌动蛋白连接到早期内体，并且在FHF复合物内，钩与动力蛋白-动力肌动蛋白相互作用，并且钩-早期内体相互作用依赖于FHIP和FTS。
Microtubule-based distribution of organelles/vesicles is crucial for the function of many types of eukaryotic cells and the molecular motor cytoplasmic dynein is required for transporting a variety of cellular cargos toward the microtubule minus ends. Early endosomes represent a major cargo of dynein in filamentous fungi, and dynein regulators such as LIS1 and the dynactin complex are both required for early endosome movement. In fungal hyphae, kinesin-3 and dynein drive bi-directional movements of early endosomes. Dynein accumulates at microtubule plus ends; this accumulation depends on kinesin-1 and dynactin, and it is important for early endosome movements towards the microtubule minus ends. The physical interaction between dynein and early endosome requires the dynactin complex, and in particular, its p25 component. The FTS-Hook-FHIP (FHF) complex links dynein-dynactin to early endosomes, and within the FHF complex, Hook interacts with dynein-dynactin, and Hook-early endosome interaction depends on FHIP and FTS.