Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse
Proteomic profiling of lipid droplet-associated proteins in primary adipocytes of normal and obese mouse
复制标题
正常和肥胖小鼠原代脂肪细胞中脂滴相关蛋白的蛋白质组学分析。
DOI：
10.1093/abbs/gms008
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发表时间：
2012-05-01
影响因子：
3.7
通讯作者：
Liao, Kan
中科院分区：
文献类型：
--
作者：
Ding, Yubo;Wu, Yibo;Liao, Kan
脂肪细胞中的脂滴是动物主要的长期能量储存库。越来越多的人认识到，脂滴不仅仅是一个静态的中性脂质储存场所，而是一个动态的多功能细胞器。在结构上，脂滴由被磷脂单层包围的中性脂核和嵌入或结合在磷脂层上的蛋白质组成。脂滴表面的蛋白质对脂滴的结构和动力学至关重要。为了解具有大中心脂滴的原代脂肪细胞脂滴相关蛋白质组，分离C57BL/6小鼠白色脂肪组织脂滴。采用液相色谱-串联质谱法对蛋白质进行提取和分析。共鉴定出193种蛋白质，其中包括73种以前未报道的蛋白质。此外，采用同位素编码亲和标签（ICAT）比较了正常瘦小鼠和高脂饮食诱导的肥胖C57BL/6小鼠脂滴相关蛋白质组的差异。在ICAT定量的23个蛋白中，肥胖小鼠脂肪组织脂滴中有3个蛋白表达上调，4个蛋白表达下调。重要的是，肥胖小鼠脂肪组织脂滴中的两种脂滴结构蛋白perilipin A和vimentin大幅减少，暗示脂滴稳定性的蛋白质机制降低。
Lipid droplets in adipocytes serve as the principal long-term energy storage depot of animals. There is increasing recognition that lipid droplets are not merely a static neutral lipid storage site, but in fact dynamic and multi-functional organelles. Structurally, lipid droplet consists of a neutral lipid core surrounded by a phospholipid monolayer and proteins embedded in or bound to the phospholipid layer. Proteins on the surface of lipid droplets are crucial to droplet structure and dynamics. To understand the lipid droplet-associated proteome of primary adipocyte with a large central lipid droplet, lipid droplets of white adipose tissue from C57BL/6 mice were isolated. And the proteins were extracted and analyzed by liquid chromatography coupled with tandem mass spectrometry. A total of 193 proteins including 73 previously unreported proteins were identified. Furthermore, the isotope-coded affinity tags (ICAT) was used to compare the difference of lipid droplet-associated proteomes between the normal lean and the high-fat diet-induced obese C57BL/6 mice. Of 23 proteins quantified by ICAT analysis, 3 proteins were up-regulated and 4 proteins were down-regulated in the lipid droplets of adipose tissue from the obese mice. Importantly, two structural proteins of lipid droplets, perilipin A and vimentin, were greatly reduced in the lipid droplets of the adipose tissue from the obese mice, implicating reduced protein machinery for lipid droplet stability.